| Ceramide from sphingomyelin hydrolysis differentially mediates mitogen-activated protein kinases （MAPKs） activation following cerebral ischemia in rat hippocampal CA1 subregion |
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| 作者姓名： | Xian Sun Chao Liu Min Qian Zhenghong Zhao Jun Guo |
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| 作者单位： | [1]The Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China [2]Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210029, China |
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| 基金项目： | supported by grants from the National Natural Science Foundation of China (No.30871200);;the Practice and Innovation Training Program for Students in Colleges and Universities of Jiangsu Province (NO.20090370) |
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| 摘 要： | Objective： To explore the role that ceramide plays in the activation of mitogen-activated protein kinases （MAPKs） during cerebral ischemia and reperfusion. Methods： Rats were subjected to ischemia by the fourvessel occlusion （4-VO） method. The sphingomyelinase inhibitor TPCK was administered to the CA1 subregion of the rat hippocampus before inducing ischemia. Western blot was used to examine the activity of extracellular- signal regulated kinase （ERK） and c-Jun N-terminal protein kinase （JNK） using antibodies against ERK, JNK and diphosphorylated ERK and JNK. Results： At lh reperfusion post-ischemia, JNK reached its peak activity while ERK was undergoing a sharp inactivation （P 〈 0.05）. The level of diphosphorylated JNK was significantly reduced but the sharp inactivation of ERK was visibly reversed （P 〈 0.05） by the sphingomyelinase inhibitor. Conclusion： The ceramide signaling pathway is up-regulated through sphingomyelin hydrolysis in brain ischemia, promoting JNK activation and suppressing ERK activation, culminating in the ischemic lesion.
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| 关 键 词： | 丝裂原活化蛋白激酶 脑缺血再灌注 海马CA1区 神经酰胺 大鼠 水解 有丝分裂原活化蛋白激酶 激活 |
Ceramide from sphingomyelin hydrolysis differentially mediates mitogen-activated protein kinases (MAPKs) activation following cerebral ischemia in rat hippocampal CA1 subregion |
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| Xian Sun,Chao Liu,Min Qian,Zhenghong Zhao,Jun Guo.Ceramide from sphingomyelin hydrolysis differentially mediates mitogen-activated protein kinases (MAPKs) activation following cerebral ischemia in rat hippocampal CA1 subregion[J].Acta Universitatis Medicinalis Nanjing,2010(2):132-137. |
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| Authors: | Xian Sun Chao Liu Min Qian Zhenghong Zhao and Jun Guo |
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| Institution: | Xian Suna,b,Chao Liua,Min Qiana,Zhenghong Zhaoa,Jun Guoa,b aThe Laboratory Center for Basic Medical Sciences,Nanjing Medical University,Nanjing 210029,China bDepartment of Biochemistry , Molecular Biology,Nanjing,210029,China |
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| Abstract: | Objective:To explore the role that ceramide plays in the activation of mitogen-activated protein kinases (MAPKs) during cerebral ischemia and reperfusion. Methods: Rats were subjected to ischemia by the four-vessel occlusion (4-VO) method. The sphingomyelinase inhibitor TPCK was administered to the CA1 subregion of the rat hippocampus before inducing ischemia. Western blot was used to examine the activity of extracellular-signal regulated kinase (ERK) and c-Jun N-terminal protein kinase (JNK) using antibodi... |
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| Keywords: | ceramide cerebral ischemia extracellular-signal regulated kinase c-Jun N-terminal protein kinase |
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