胃癌组织中MMP-14蛋白的表达及在腹腔转移中的临床意义

目的:探讨MMP-14蛋白在胃癌组织中的表达及在腹腔转移中的临床意义?方法:应用RT-PCR法检查98例原发性胃癌腹腔冲洗液中MMP-14的表达,30例良性病变患者腹腔冲洗液作为阴性对照?免疫组织化学SP法检测98例原发性胃癌和20例正常胃黏膜组织MMP-14表达?结果:腹腔冲洗液中MMP-14阳性表达率分别为40.8% (40/98),高于对照组的3.33% (1/30)(P < 0.01)?胃癌组织中MMP-14的阳性表达率为76.5%(58/98),显著高于正常胃黏膜组织的5%(1/20)(P < 0.01)?MMP-14在胃癌组织中的阳性表达率与患者的性别?年龄?分化程度无相关性(P > O.05),而MMP-14的阳性表达率与胃癌的肿瘤浸润深度?肿瘤大小?淋巴转移?大体分型?临床肿瘤分期有关(P < 0.05,P < 0.01)?结论:胃癌组织中MMP-14高表达,结合在腹腔冲洗液中MMP-14 mRNA的高表达,MMP-14参与了胃癌的发生发展和腹腔转移的过程?

The expression of MMP-14 in gastric carcinoma and its clinical significance in peritoneal metastasis

Objective:To investigate the expression and clinical significance of MMP-14 in gastric carcinoma and its role in peritoneal metastasis. Methods:The mRNA expression of MMP-14 in peritoneal washing samples were deteced by RT-PCR in gastric carcinoma(98 cases) and normal control(30 cases). Immunohistochemical staining S-P method was used to detect the expression of MMP-14 in gastric carcinoma(98 cases) and normal gastric mucosa(20 cases). Results:The expression rates of MMP-14 were 76.5%(58/98) in gastric cancer tissues and 5%(1/20)in normal gastric mucosa tissues,which were significantly higher than those in the normal tissues respectively (P < 0.01). The MMP-14 mRNA positive rate of peritoneal washing samples in gastric cancer was higher than that in normal control. The positive expression rate of MMP14 was related to the depth of invasion,the size of tumor,lymph node metastasis,macroscopic type and clinial stage (P < 0.05,0.01),but not related to age,gender and differentiation of tumor(P > O.05). Conclusion:The high expression of MMP-14 in gastric carcinoma and peritoneal washing samples suggests that MMP-14 may be involved in the development of gastric cancer and peritoneal metastasis process.