| HSV-1作用于KSHV ORF50启动子区中特异性应答位点序列的初寻 |
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| 引用本文： | 程 伟,郝婷婷,王子盾,朱小飞,冯宁翰,华立新,秦 娣,卢 春.HSV-1作用于KSHV ORF50启动子区中特异性应答位点序列的初寻[J].南京医科大学学报,2011(5):595-600. |
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| 作者姓名： | 程 伟 郝婷婷 王子盾 朱小飞 冯宁翰 华立新 秦 娣 卢 春 |
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| 作者单位： | 南京医科大学第一附属医院泌尿外科;南京医科大学微生物学与免疫学系; |
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| 基金项目： | 国家自然科学基金(30900064); 教育部高等学校博士点专项科研基金新教师基金(20093234120004); 江苏省高校自然科学基金(09KJB310007); 江苏省卫生厅医学重点学科(实验室)开发课题(09hx41); 南京医科大学科技发展基金(08NMUM009) |
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| 摘 要： | 目的:构建卡波济肉瘤相关疱疹病毒(Kaposi's sarcoma-associated herpesvirus,KSHV)ORF50启动子系列截短序列克隆,初步寻找HSV-1作用于KSHV ORF50启动子区中特异性应答位点序列.方法:以KSHV基因组为模板,PCR扩增KSHVORF50启动子系列截短序列,克隆至含有...
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| 关 键 词： | KSHV HSV-1 ORF50启动子 虫荧光素酶 |
| 收稿时间： | 2010/11/1 0:00:00 |
The detection of the specific sequence of the promoter region of the KSHV ORF50 acted by HSV-1 |
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| CHENG Wei,HAO Ting-ting,WANG Zi-dun,ZHU Xiao-fei,FENG Ning-han,HUA Li-xin,QIN Di and LU Chun.The detection of the specific sequence of the promoter region of the KSHV ORF50 acted by HSV-1[J].Acta Universitatis Medicinalis Nanjing,2011(5):595-600. |
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| Authors: | CHENG Wei HAO Ting-ting WANG Zi-dun ZHU Xiao-fei FENG Ning-han HUA Li-xin QIN Di and LU Chun |
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| Institution: | CHENG Wei,HAO Ting-ting1,WANG Zi-dun,ZHU Xiao-fei1,FENG Ning-han,HUA Li-xin,QIN Di1,LU Chun1(Department of Urology,the First Affiliated Hospital of NJMU,Nanjing 210029,1Department of Microbiology and Immunology,NJMU,China) |
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| Abstract: | Objective:To construct the recombinant luciferase reporter plasmids containing series of truncated sequences of Kaposi's sarcoma-associated herpesvirus(KSHV) ORF50 promoter and seek the specific response sites sequences of ORF50 promoter acted by HSV-1.Methods:Series of truncated sequences of KSHV ORF50 promoter were amplified using PCR from KSHV genome.The PCR products were further cloned into pGL3 basic vector to construct the recombinant luciferase reporter plasmids.The recombinant plasmids were confirme... |
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| Keywords: | KSHV HSV-1 ORF50 promoter luciferase |
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