PNPLA7在脂肪组织中的表达及调控的初步研究

目的：明确PNPLA7在小鼠不同组织及营养状态下的表达水平；探索不同因素对PNPLA7表达的调节作用。方法：利用生物信息学初步分析PNPLA7在正常组织器官及细胞系中的表达，荧光定量PCR的方法进一步验证PNPLA7在小鼠不同组织及不同营养状态下的表达情况。免疫印迹法及荧光定量PCR法检测脂肪细胞分化过程中和脂肪细胞在胰岛素及脂肪酸处理下PNPLA7表达水平的变化。结果：PNPLA7主要表达于代谢旺盛的组织或细胞中。在饥饿条件下，小鼠脂肪组织PNPLA7的表达水平比正常饮食条件下高，但小鼠饥饿后又喂食，其脂肪组织PNPLA7的表达水平又降低。脂肪细胞中PNPLA7表达在胰岛素处理时下调，油酸处理时上调。且在脂肪细胞分化过程中，PNPLA7表达前期下降，后期逐渐升高。结论：PNPLA7在脂肪组织中的表达水平较高，脂肪细胞分化过程中其表达水平先下降后升高，且受胰岛素及不饱和脂肪酸调控。

The expression and regulation of PNPLA7 in adipocyte

Objective: To explore distributions of PNPLA7 in different tissues of mouse under different nutritional status and its expression levels in the process of adipocyte differentiation as well as insulin or oil acids treatment on adipocytes. Methods: The distributions of PNPLA7 in different tissues and cell lines were preliminarily analyzed by bioinformatics and to further identify the expression of PNPLA7 in different tissues of mouse under different nutritional status by real time PCR. In addition, 3T3-L1 cell was induced differentiation and then detected the PNPLA7 expression in the process of differentiation by real time PCR and western blot as well as detected its expression in mature adipocytes treated with insulin or oil acids. Results: PNPLA7 was mainly expressed in active metabolic tissues or cells. The expression level of PNPLA7 in adipocytes under starvation was higher than that in normal diet, while decreased in re-feedingSafterSstarvation status. Besides, the expression of PNPLA7 increased in the process of differentiation of 3T3-L1 cell as well as decreased in insulin treatment while increased in oil acids treatment. Conclusions: PNPLA7 was enriched in adipocytes and was increased during adipocyte differentiation Besides, its expression was regulated by insulin and oil acids. Taken together, our results provided a basis for further study of the role of PNPLA7 in adipocytes.