| 幽门螺杆菌CagA基因的克隆 |
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| 引用本文： | 李崇勇,张一鸣,徐顺福.幽门螺杆菌CagA基因的克隆[J].南京医科大学学报,2000,20(6):423-424. |
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| 作者姓名： | 李崇勇 张一鸣 徐顺福 |
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| 作者单位： | 南京医科大学生物化学教研室,南京 |
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| 基金项目： | 江苏省科委社会发展基金项目 ( BS970 3 5 ) |
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| 摘 要： | 目的 为在大肠杆菌表达幽门螺杆菌ＣａｇＡ蛋白，建立ＥＬＩＳＡ法检测ＣａｇＡ抗体，克隆幽门螺杆菌ｃａｇＡ基因片段。方法 根据ｃａｇＡ基因序列，设计引物ＰＣＲ扩增ｃａｇＡ基因片段，用Ｔ－Ａ克隆的方法将ＰＣＲ产物克隆入质粒载体ｐＧＥＭ－Ｔ，并转化大肠杆菌ＪＭＩ１０９。结果 经蓝白斑筛选，ＰＣＲ及限制性内切酶酶切分析，获得ｃａｇＡ基因片段的克隆，序列测定结果与文献报道一致。结论 ＰＣＲ产物可采用Ｔ－Ａ法克
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| 关 键 词： | 幽门螺杆菌 基因克降 cag基因 胃疾病 |
| 修稿时间： | 2000年5月10日 |
Cloning of cagA Fragment of Helicobacter Pylori |
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| Li Congyong,Zhang Yiming,Xu Shunfu.Cloning of cagA Fragment of Helicobacter Pylori[J].Acta Universitatis Medicinalis Nanjing,2000,20(6):423-424. |
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| Authors: | Li Congyong Zhang Yiming Xu Shunfu |
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| Abstract: | Objective To prepare the products of cagA gene of helicobacter pylori. Methods The primers were designed according to the sequence of cagA gene, and the cagA gene fragment was amplified by PCR and transfected into E.coli through TA strategy with pGEM T vector .Results The recombinant clones were tested by the methods of restrictional enzyme cut and PCR , the sequence of cagA gene was accordant with other reports.Conclusion The recombinant clones may be used in the further studies. |
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| Keywords: | helicobacter pylori cagA gene cloning |
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