尿道下裂大鼠与正常大鼠睾丸蛋白质二维电泳图谱分析

目的:探讨二维电泳技术在尿道下裂大鼠与正常大鼠睾丸差异表达蛋白研究中的应用.方法:孕SD大鼠10只,随机分为2组,妊娠12～19天,实验组和对照组分别予邻苯二甲酸二丁酯(DBP)500mg/(kg·d)、大豆油5 ml/d灌胃,第2l天取出仔鼠睾丸,提取总蛋白,进行二维凝胶电泳分离和图像分析.结果:按照5倍的表达量计算,发现有差异表达的蛋白质点9个,5个点在尿道下裂大鼠睾丸中高表达,而在正常大鼠睾丸为低表达;相反,在正常大鼠睾丸中高表达的4个点,在尿道下裂者为低表达.结论:初步建立了正常和尿道下裂大鼠睾丸的二维电泳图谱,并发现两者之间存在一些差异表达蛋白,为进一步进行尿道下裂大鼠睾丸差异表达蛋白的分离及鉴定奠定了良好基础.

Differential analysis of two-dimensional gel electrophoresis profiles of testis protein in normal and hypospadiac male rats

Objective:To isolate and identify differential expression proteins in embryonic rat testis after maternal exposure to di-nbutyl-phthalate(DBP). Methods:Two-dimensional gel electrophoresis was performed on testes samples from hypospadiac and normal healthy rats. After silver staining,the differential expression proteins were analyzed by ImageMaster 2D analysis software. Results:Nine differential protein spots were found,calculated according to 5-fold expression amount. Five expressed highly in the hypospadiac rat testis but lowly in the normal rat testis,while the other four expressed just the opposite way. Conclusion:The protein profiles of differential expression between the normal and hypospadiac rat testes were established,and some differential proteins were found. The data obtained in this study might be helpful for further studies on the isolation and identification of differentially expressed proteins in hypospadiac rat testes.