TMB-8体外对神经细胞保护作用及其机理的研究

目的 研究ＴＭＢ－８［８－（Ｎ，Ｎ－二乙胺）－ｎ－辛基－３，４，５－三甲氧基苯甲酸酯］的神经保护作用及其可能机制。方法 用氧－葡萄糖剥夺模型观察ＴＭＢ－８对培养的大鼠神经细胞形态、细胞膜通透性和细胞内丙二醛（ＭＤＡ）、总过氧化物歧化酶（Ｔ－ＳＯＤ）、谷胱甘肽（ＧＳＨ）和谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ）的影响。结果 经氧－葡萄糖剥夺１ｈ后，无血清培养２４ｈ的神经细胞乳酸脱氢酶（ＬＤＨ）漏出明显增加

Protective Effects and Mechanism of TMB-8 on Neuron

Objective To investigate the neuroprotective effect and mechanism of TMB 88 (N, N diethylamino) n octyl 3,4,5 trimethoxybenzoate].Methods The oxygen glucose deprivation model of cultured rat neuron was used to observe the effects of TMB 8 on neuron morphology, permeability and contents of MDA, GSH, T SOD and GSH px in neurons.Results The damaged neurons induced by oxygen glucose deprivation for 1 h and after serum free culture for 24 h could liberate much LDH; the contents of GSH and the activity of T SOD in neurons were significantly decreased; TMB 8 could concentration dependently reduce the efflux of LDH from the damaged neuron and increase the content of GSH in neuron, the activity of T SOD in these cells was also increased.Conclusion the neuroprotective role of TMB 8 may be related to the increasing of GSH and T SOD in the damaged neuron.