| 小电导型钙激活钾通道在自发性高血压大鼠阴茎海绵体中的表达 |
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| 引用本文： | 廖 胜,高 飞,葛余正,于 鹏,赵 炎,吴 然,吴剑平,贾瑞鹏.小电导型钙激活钾通道在自发性高血压大鼠阴茎海绵体中的表达[J].南京医科大学学报,2014(10):1305-1307. |
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| 作者姓名： | 廖 胜 高 飞 葛余正 于 鹏 赵 炎 吴 然 吴剑平 贾瑞鹏 |
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| 作者单位： | 南京医科大学附属南京医院泌尿外科,江苏 南京 210006;南京医科大学附属南京医院泌尿外科,江苏 南京 210007;南京医科大学附属南京医院泌尿外科,江苏 南京 210008;南京医科大学附属南京医院泌尿外科,江苏 南京 210009;南京医科大学附属南京医院泌尿外科,江苏 南京 210010;南京医科大学附属南京医院泌尿外科,江苏 南京 210011;南京医科大学附属南京医院泌尿外科,江苏 南京 210012;南京医科大学附属南京医院泌尿外科,江苏 南京 210013 |
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| 基金项目： | 国家自然科学基金(81070597,81370853);江苏省卫生厅科教兴卫工程领军人才与创新团队科研课题(LJ201107);江苏省六大人才高峰资助项目(2011-WS-093);江苏省科教兴卫工程重点学科开放课题(KF200942) |
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| 摘 要： | 目的:探讨高血压对小电导型钙激活钾通道(SKCa)在大鼠阴茎海绵体中表达的影响及意义?方法:选用同龄雄性自发性高血压大鼠(SHR)及同系正常血压大鼠(WKY)各8只,10%利多卡因麻醉后连续检测海绵体内压(ICP)及平均动脉压(MAP),并用5 V电刺激海绵体神经(CN)记录ICP/MAP值变化?运用Western blot 和逆转录-聚合酶链式反应(RT-PCR)技术检测阴茎海绵体组织中SKCa蛋白和mRNA表达?结果:5 V电刺激后SHR组ICP/MAP值显著低于WKY组(0.31 ± 0.04 vs 0.76 ± 0.06),SKCa蛋白和mRNA的表达在SHR组(0.18 ± 0.01?0.39 ± 0.01)较WKY组(0.49 ± 0.02?0.81 ± 0.08)明显降低,差异具有统计学意义(P < 0.05)?结论:高血压可以降低大鼠勃起功能,这可能与自发性高血压大鼠阴茎海绵体组织中SKCa表达下降密切相关?
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| 关 键 词： | 自发性高血压大鼠 勃起功能障碍 阴茎海绵体 钾通道蛋白 |
| 收稿时间： | 5/6/2014 12:00:00 AM |
SKCa in cavernous tissue of spontaneously hypertensive rats |
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| Liao Sheng,Gao Fei,Ge Yuzheng,Yu Peng,Zhao Yan,Wu Ran,Wu Jianping and Jia Ruipeng.SKCa in cavernous tissue of spontaneously hypertensive rats[J].Acta Universitatis Medicinalis Nanjing,2014(10):1305-1307. |
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| Authors: | Liao Sheng Gao Fei Ge Yuzheng Yu Peng Zhao Yan Wu Ran Wu Jianping and Jia Ruipeng |
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| Institution: | Department of Urinary, Nanjing Hospital Affiliated to NJMU, Nanjing 210006, China;Department of Urinary, Nanjing Hospital Affiliated to NJMU, Nanjing 210007, China;Department of Urinary, Nanjing Hospital Affiliated to NJMU, Nanjing 210008, China;Department of Urinary, Nanjing Hospital Affiliated to NJMU, Nanjing 210009, China;Department of Urinary, Nanjing Hospital Affiliated to NJMU, Nanjing 210010, China;Department of Urinary, Nanjing Hospital Affiliated to NJMU, Nanjing 210011, China;Department of Urinary, Nanjing Hospital Affiliated to NJMU, Nanjing 210012, China;Department of Urinary, Nanjing Hospital Affiliated to NJMU, Nanjing 210013, China |
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| Abstract: | Objective:To validate the effect of hypertension on small conductance calcium-activated potassium channels in cavernous tissue of spontaneously hypertensive rats. Methods: Male spontaneously hypertensive rats (SHR) (n = 8) and normotensive Wistar Kyoto rats (WKY) (n = 8) were selected. A series of electric stimulation were utilized to the corpus cavernosum nerves of rats, the changes of ICP /MAP were observed repeatedly, and the expressions of SKCa protein and mRNA were assessed by Western blotting and RT-PCR. Results: Compared with the WKY group, ICP /MAP and the expression of SKCa gene mRNA and protein were significantly lower in the SHR group (P < 0.05), and the expression of SKCa protein and mRNA was significantly lower in the SHR group in comparison with that of the WKY group (P < 0.05). Conclusion: Erectile dysfunction in hypertension is associated with the low expression of SKCa in the cavernous tissue of spontaneously hypertensive rats. |
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| Keywords: | spontaneously hypertensive rats erectile dysfunction corpus cavernosum penis potassium channel protein |
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