胰岛素对高糖条件下人脐静脉内皮细胞凋亡及小窝蛋白-1表达的影响

目的:探讨不同浓度的胰岛素对高糖条件下人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)凋亡及小窝蛋白(caveolin,Cav)-1表达的影响?方法:体外培养HUVEC,分为A组(对照组,葡萄糖浓度5.5 mmol/L)?B组(高糖组,葡萄糖浓度33.3 mmol/L)?C组(高糖+低浓度胰岛素组,葡萄糖浓度33.3 mmol/+胰岛素浓度20 mU/L)?D组(高糖+高浓度胰岛素组,葡萄糖浓度33.3 mmol/L+胰岛素浓度1 000 mU/L)?采用流式细胞仪检测细胞凋亡比例及细胞周期,免疫组化检测HUVEC的Cav-1表达?结果:在高糖条件下,HUVEC凋亡比例显著升高,停滞于G0/G1期细胞增加,Cav-1表达减少;低浓度胰岛素使细胞凋亡比例减少,Cav-1表达增加;加入高浓度胰岛素不能改变细胞凋亡比例及细胞周期,Cav-1表达亦无明显改变?结论:低浓度胰岛素可抑制高糖导致的内皮细胞凋亡,而高浓度胰岛素无此作用,其机制可能与Cav-1表达有关?

Effects of insulin on apoptosis of cultured human umbilical vein endothelial cells(HUVECs)and expression of caveolin in HUVECs

Objective:To detect the effect of insulin on apoptosis of cultured human umbilical vein endothelial cells (HUVECs) induced by hyperglycemia and the expression of caveolin-1 in HUVECs. Methods:HUVECs were cultured and randomized into group A(the controlled group,glucose concentration 5.5 mmol/L),group B (the hyperglucose group,glucose concentration 33.3 mmol/L),group C(the hyperglucose and low concentration of insulin group,glucose concentration 33.3 mmol/L+insulin concentration 20 mU/L),group D (the hyperglucose and high concentration of insulin group,glucose concentration 33.3 mmol/L+insulin concentration 1 000 mU/L). Flow cytometry was applied to determine cell apoptosis ratio and cell cycle. Cav-1 expression of HUVEC was detected by immunohistochemistry analysis. Results:High concentration of glucose induced sighificantly increased apoptosis of HUVEC which was associated with the arrest of the cell cycle of G0/G1, and the expression of cav-1 was decreased. Low concentration of insulin reduced the apoptosis of HUVEC and increased cav-1 expression. While high concentration of insulin had no effects on the cells apoptosis,cell cycle and cav-1 expression. Conclusions:Low concentration of insulin inhibited the apoptosis induced by hyperglycemia,while high concentration of insulin had no effects on the apoptosis induced by hyperglycemia, and its mechanism may be related to the expression of cav-1.