| 康复训练对脊髓损伤后大鼠脊髓内再生微环境的影响 |
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| 引用本文： | 雷晓婷,刘兴波,王红星,徐冬晨,王 彤,丁新生.康复训练对脊髓损伤后大鼠脊髓内再生微环境的影响[J].南京医科大学学报,2009,29(4):429-434. |
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| 作者姓名： | 雷晓婷 刘兴波 王红星 徐冬晨 王 彤 丁新生 |
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| 作者单位： | 雷晓婷,LEI Xiao-ting(江苏省中医院医务处,江苏南京,210029);刘兴波,LIU Xing-bo(南京医科大学第二附属医院神经内科,江苏南京,210011);王红星,徐冬晨,王彤,WANG Hong-xing,XU Dong-chen,WANG Tong(南京医科大学第一附属医院康复科,江苏南京,210029);丁新生,DING Xin-sheng(南京医科大学第一附属医院神经内科,江苏南京,210029)
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| 摘 要： | 目的:观察运动训练对脊髓损伤后大鼠运动功能的影响,以及脊髓内脑源性神经营养因子(BDNF)、诱导轴突分化因子Slit2、胶质纤维酸性蛋白(GFAP)的表达规律,研究康复训练对损伤后脊髓微环境的影响.方法:雄性成年SD大鼠70只,随机分为10个实验组和4个对照组,每组5只.以改良Allen's撞击法制作脊髓损伤模型.从损伤后7天起,实验组分别进行1-4周活动平板训练,对照组不训练.训练结束时(即损伤后14、21、28、35天)分别进行运动功能评估,免疫组织化学二步法结合图像平均光密度分析观察大鼠脊髓内BDNF、Slit2以及GFAP的表达.结果:各不同训练时程后,大鼠运动功能评分均有改善(P<0.05);训练后,大鼠脊髓内BDNF以及Slit2的表达较对照组均有明显增加(P<0.05),GFAP的表达高峰延迟.结论:康复训练可能通过上调脊髓内BDNF和Slit2的表达水平,延迟星型胶质细胞的增生,使脊髓内神经元再生微环境朝有利方向变化来促进神经通路重建,改善运动功能.
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| 关 键 词： | 康复训练 脊髓损伤 神经再生 脑源性神经营养因子 |
| 收稿时间： | 2008/11/13 0:00:00 |
Effect of rehabilitation training on nerve regeneration microenviroment after spinal cord injury of rats |
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| LEI Xiao-ting,LIU Xing-bo,WANG Hong-xing,XU Dong-chen,WANG Tong and DING Xin-sheng.Effect of rehabilitation training on nerve regeneration microenviroment after spinal cord injury of rats[J].Acta Universitatis Medicinalis Nanjing,2009,29(4):429-434. |
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| Authors: | LEI Xiao-ting LIU Xing-bo WANG Hong-xing XU Dong-chen WANG Tong and DING Xin-sheng |
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| Institution: | Department of Neurology;the First Affiliated Hospital of NJMU;Nanjing 210029;1Department of Neurology;the Second Affiliated Hospital of NJMU;Nanjing 210011;2Department of Physiatry;Naning 210029;China |
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| Abstract: | Objective:To investigate the effect of movement training on the motor function repair of rats with a spinal cord contusion,and to simultaneously observe BDNF,Slit2 and GFAP expression of rats.Methods:Seventy male adult Sprague Dawley rats were randomly divided into 10 training groups and 4 control groups,each group with 5 rats.Moderate spinal cord contusive injuries were produced by using modified Allen's impactor method to all experimental rats.After injury,rats in training groups were exposed to the tread... |
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| Keywords: | Slit2 |
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