| 江苏省113株大肠杆菌O157:H7的扩增片段长度多态性分析 |
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| 引用本文： | 张平平,朱叶飞,董〓晨,钱慧敏,朱凤才.江苏省113株大肠杆菌O157:H7的扩增片段长度多态性分析[J].南京医科大学学报,2010(1):134-138. |
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| 作者姓名： | 张平平 朱叶飞 董〓晨 钱慧敏 朱凤才 |
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| 作者单位： | 东南大学公共卫生学院,江苏 南京〓210009;江苏省疾病预防控制中心,江苏 南京〓210024;江苏省疾病预防控制中心,江苏 南京〓210024;江苏省疾病预防控制中心,江苏 南京〓210024;江苏省疾病预防控制中心,江苏 南京〓210024 |
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| 基金项目： | 国家高技术研究发展计划（863）资助（2007AA022409） |
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| 摘 要： | 目的:分析江苏省不同地区和年代分离的Escherichia coli O157:H7(E. coli O157:H7)之间的同源性?方法:用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)分析法对113株 E. coli O157:H7菌株进行分子分型,采用软件BioNumerics Version 4.0对分型数据进行处理和分析?结果:113株E. coli O157:H7共分37个型别,不同来源的菌株存在相同的基因型别,不同的地区和物种之间存在着相互传播?结论:AFLP可以用于对不同来源的E. coli O157:H7进行分子分型?
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| 关 键 词： | E. coli O157:H7 AFLP 分子分型 |
| 收稿时间： | 7/2/2009 12:00:00 AM |
Amplified fragment length polymorphism of 113 enterohemorrhagic Escherichia coli O157:H7 isolated in Jiangsu Province |
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| ZHANG Ping-ping,ZHU Ye-fei,DONG Chen,QIAN Hui-min and ZHU Feng-cai.Amplified fragment length polymorphism of 113 enterohemorrhagic Escherichia coli O157:H7 isolated in Jiangsu Province[J].Acta Universitatis Medicinalis Nanjing,2010(1):134-138. |
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| Authors: | ZHANG Ping-ping ZHU Ye-fei DONG Chen QIAN Hui-min and ZHU Feng-cai |
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| Institution: | Institute of Public Health,Southeast University,Nanjing 210009;Jiangsu Provincial Center for Disease Control and Preventio,Nanjing 210024,China;Jiangsu Provincial Center for Disease Control and Preventio,Nanjing 210024,China;Jiangsu Provincial Center for Disease Control and Preventio,Nanjing 210024,China;Jiangsu Provincial Center for Disease Control and Preventio,Nanjing 210024,China |
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| Abstract: | Objective:To identify the homology among enterohemorrhagic Escherichia coli (EHEC) isolated from different years,cites and hosts. Methods:Amplified fragment length polymorphism (AFLP) was used for the molecular subtyping of 113 E. coli O157:H7 strains. The AFLP patterns were analyzed by BioNumerics version 4.0 software. Results:113 isolates showed 37 patterns. Strains isolated from different years and hosts may have the same patterns. Conclusion:From the above observations,it is evident that AFLP is useful in the molecular typing of E. coli O157:H7 isolated from different sources. |
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| Keywords: | E coli O157:H7 amplified fragment length polymorphism molecular subtyping |
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