| 肾母细胞瘤过表达基因促进肝癌细胞的侵袭与转移 |
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| 引用本文： | 卢叶挺,丁文周,于建军,谭龙威,孔连宝.肾母细胞瘤过表达基因促进肝癌细胞的侵袭与转移[J].南京医科大学学报,2015(8):1087-1091. |
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| 作者姓名： | 卢叶挺 丁文周 于建军 谭龙威 孔连宝 |
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| 作者单位： | 南京医科大学第一附属医院肝脏外科,江苏 南京 210029,南京医科大学第一附属医院肝脏外科,江苏 南京 210029,南京医科大学第一附属医院肝脏外科,江苏 南京 210029,南京医科大学第一附属医院肝脏外科,江苏 南京 210029,南京医科大学第一附属医院肝脏外科,江苏 南京 210029 |
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| 基金项目： | 卫生部医药发展项目(W2012Fz058) |
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| 摘 要： | 目的:探讨肾母细胞瘤过表达基因(NOV CCN3)在肝癌中的表达及在肝癌侵袭中的作用?方法:荧光定量PCR(real-time PCR)检测肝癌及癌旁组织中的CCN3的mRNA表达水平,同时检测肝癌细胞系及正常人肝细胞中CCN3的mRNA表达水平,Western blot检测肝癌及癌旁组织中CCN3蛋白的表达量;通过肝癌细胞过表达CCN3分析CCN3蛋白对肝癌细胞转移的作用;运用Transwell侵袭实验和划痕迁移实验研究CCN3过表达对肝癌细胞侵袭和迁移能力的影响?结果:50对样本中有39对CCN3的mRNA表达水平在肝癌组织中高于癌旁组织,Western blot提示肝癌组织中CCN3蛋白表达量高于癌旁组织?体外肝癌细胞的功能试验中,过表达CCN3能促进MHCC-97H?SMMC-7721细胞的侵袭与转移?结论:CCN3在肝癌组织中高表达,过表达CCN3能促进肝癌的侵袭与转移,CCN3发挥作用机制的研究能为肝细胞肝癌的治疗提供新的思路?
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| 关 键 词： | CCN3 肝细胞肝癌 肿瘤侵袭 |
| 收稿时间： | 2015/3/13 0:00:00 |
Nephroblastoma overexpressed gene promotes migration and invasion of hepatocellular carcinoma |
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| Lu Yeting,Ding Wenzhou,Yu Jianjun,Tan Longwei and Kong Lianbao.Nephroblastoma overexpressed gene promotes migration and invasion of hepatocellular carcinoma[J].Acta Universitatis Medicinalis Nanjing,2015(8):1087-1091. |
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| Authors: | Lu Yeting Ding Wenzhou Yu Jianjun Tan Longwei and Kong Lianbao |
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| Institution: | Department of Liver Transplantation Center,the First Affiliated Hospital of NJMU,Nanjing 210029,China,Department of Liver Transplantation Center,the First Affiliated Hospital of NJMU,Nanjing 210029,China,Department of Liver Transplantation Center,the First Affiliated Hospital of NJMU,Nanjing 210029,China,Department of Liver Transplantation Center,the First Affiliated Hospital of NJMU,Nanjing 210029,China and Department of Liver Transplantation Center,the First Affiliated Hospital of NJMU,Nanjing 210029,China |
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| Abstract: | Objective:To detect the expression and study the role of nephroblastoma overexpressed gene (NOV CCN3)in hepatocelluar carcinoma(HCC). Methods:The expressions of CCN3 mRNA in HCC samples and cell lines were detected by real-time PCR,and the expressions of CCN3 protein in HCC tissues and adjacent non-cancer tissues were assessed by Western blot. In addition,invasion of HCC cells was observed after overexpressing CCN3 by Transwell invasion assay and wound healing assay. Results:In the total of 50 paired HCC specimens,compared with the adjacent non-cancer tissues,the expression of CCN3 mRNA was up-regulated in 39 cases,and the expression of CCN3 protein in HCC tissues was higher than that in adjacent non-cancer tissues. Overexpression of CCN3 enhanced HCC-derived MHCC-97H,SMMC-7721 cellular invasion in vitro. Conclusion:This study indicates CCN3 is frequently overexpressed in hepatocellular carcinoma,and contributes to tumor cell invasion. The study of CCN3 may provide a novel therapeutic idea for prevention and treatment of invasion in HCC. |
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| Keywords: | CCN3 hepatocellular carcinoma tumor invasion |
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