肾素血管紧张素系统基因多态性与冠心病关系的研究

目的：探讨中国苏皖地区人群血管紧张素转换酶（ACE）和血管紧张素原（AGT）基因型的分布及其与冠心病的关系。方法：应用聚合酶链反应技术，对106例冠心病患者、56例冠状动脉造影正常者以及30名健康献血员ACE插入／缺失（I／D）多态性及AGTM235T多态性进行检测。结果：ACE基因型分布及等位基因频率在病例组及对照组间差异无显著性。病例组和对照组AGT基因型总体分布差异亦无显著性，而病例组T等位基因频率（0．778）显著高于对照组（0．698，P＜0．05）。联合分析ACEDD型及AGT TT型罹患冠心病的相对风险，其比数比（OR）为3．61，高于单基因ACEDD型（1．23）及AGT TT型（1．77）。结论：AGTM235T基因多态性中T等位基因是中国苏皖地区人群冠心病发病的危险因素之一。同时具有ACE DD型及AGT TT型发生冠心病的相对风险显著高于单基因ACE DD型及单基因AGT TT型。

Association between Gene Polymorphism of Renin angiotensin System and Co ronary Heart Disease

Objective:To evaluate the association between the m ol ecular variants of renin angiotensin system and the risk of coronary heart dise ase (CHD) in Chinese people from Jiangsu and Anhui provinces. Methods: Insertion/deletion of angiotensin converting enzyme (ACEI/D) and angiotensinoge n (AGT) M235T polymorphisms were determined by the means of polymerase chain rea ction in 106 CHD patients and the control group, the latter was composed of 30 h ealthy subjects and 56 persons with normal results of coronary angiography. Results:T allele of AGT gene was more frequent in case group than that in control group ( P <0.05). The odds ratio (OR) estimated by combined analysis w ith AGT TT and ACE DD genotypes (3.61) was markedly increased compared with that e stimated alone from either AGT TT(1.77) or ACE DD (1.23) genotype. Concl usion:The study suggests that AGT 235T allele should be a risk factor for CH D and MI in Chinese people from Jiangsu and Anhui provinces and combined analysi s of the ACE DD and AGT TT genotype may enhance the predictability of CHD.