| 唑来膦酸对破骨细胞分化中钙调蛋白?钙调磷酸酶表达的影响 |
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| 引用本文： | 阴露佳,林珏杉,温黎明,董 伟,冯晓洁,孙 红,戚孟春.唑来膦酸对破骨细胞分化中钙调蛋白?钙调磷酸酶表达的影响[J].南京医科大学学报,2016(2):171-175. |
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| 作者姓名： | 阴露佳 林珏杉 温黎明 董 伟 冯晓洁 孙 红 戚孟春 |
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| 作者单位： | 华北理工大学口腔医学院口腔颌面外科教研室,河北 唐山 063000,华北理工大学口腔医学院口腔颌面外科教研室,河北 唐山 063000,华北理工大学口腔医学院口腔颌面外科教研室,河北 唐山 063000,华北理工大学口腔医学院口腔颌面外科教研室,河北 唐山 063000,华北理工大学口腔医学院口腔颌面外科教研室,河北 唐山 063000,华北理工大学基础医学院病理教研室,河北 唐山 063000,华北理工大学口腔医学院口腔颌面外科教研室,河北 唐山 063000 |
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| 基金项目： | 国家自然科学基金(81270965) |
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| 摘 要： | 目的:探讨唑来膦酸(zoledronate,ZOL)对破骨细胞分化中钙调蛋白(calmodulin)和钙调磷酸酶(calcineurin)基因表达的影响?方法:小鼠RAW264.7细胞分为A?B两组,均用100 ng/mL核因子κB受体激活蛋白配体(RANKL)诱导4 d;B组细胞在RANKL 诱导第2天加入1×10-6 mol/L ZOL处理48 h?RANKL 诱导4 d后收获细胞,检测破骨细胞生成及calmodulin?calcineurin基因表达情况?结果:B组多核破骨细胞数?吸收陷窝数目及面积分别为(8.8 ± 2.3)个?(6.7 ± 1.2)个和(997.1 ± 14.8)μm2,均显著低于A组的(19.7 ± 2.9)个?(13.3 ± 1.5)个和(1 676.9 ± 24.9) μm2(P < 0.01)?B组calmodulin mRNA及蛋白水平较A组分别降低了51.0%和78.7%(P < 0.05),calcineurin则分别下降了37.0%和69.5%(P < 0.01)?免疫荧光细胞化学显示,B组calmodulin?calcineurin的荧光强度较A组也明显下降?结论:ZOL可显著抑制破骨细胞生成,并下调破骨细胞分化中calmodulin和calcineurin的mRNA和蛋白水平,而calmodulin?calcineurin可能参与了ZOL对破骨细胞形成和功能的抑制?
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| 关 键 词： | 唑来膦酸 破骨细胞 钙调蛋白 钙调磷酸酶 基因调控 |
| 收稿时间： | 2015/7/23 0:00:00 |
The effect of zoledronate on gene expression of calmodulin and calcineurin during osteoclast differentiation |
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| Yin Luji,Lin Jueshan,Wen Liming,Dong Wei,Feng Xiaojie,Sun Hong and Qi Mengchun.The effect of zoledronate on gene expression of calmodulin and calcineurin during osteoclast differentiation[J].Acta Universitatis Medicinalis Nanjing,2016(2):171-175. |
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| Authors: | Yin Luji Lin Jueshan Wen Liming Dong Wei Feng Xiaojie Sun Hong and Qi Mengchun |
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| Institution: | Department of Oral and Maxillofacial Surgery,College of Stomatology,North China University of Science and Technology,Tangshan 063000,China,Department of Oral and Maxillofacial Surgery,College of Stomatology,North China University of Science and Technology,Tangshan 063000,China,Department of Oral and Maxillofacial Surgery,College of Stomatology,North China University of Science and Technology,Tangshan 063000,China,Department of Oral and Maxillofacial Surgery,College of Stomatology,North China University of Science and Technology,Tangshan 063000,China,Department of Oral and Maxillofacial Surgery,College of Stomatology,North China University of Science and Technology,Tangshan 063000,China,Department of Pathology,College of Basic Medicine,North China University of Science and Technology,Tangshan 063000,China and Department of Oral and Maxillofacial Surgery,College of Stomatology,North China University of Science and Technology,Tangshan 063000,China |
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| Abstract: | |
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| Keywords: | zoledronate osteoclast calmodulin calcineurin gene regulation |
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