新型冠状病毒实验室检测方法及应用

随着新型冠状病毒（severe acute respiratory syndrome coronavirus?2，SRAS?CoV?2）分离培养与基因组测序的完成，新型冠状病毒肺炎（corona virus disease，COVID?19）的确诊主要依靠RT?PCR 法检测SARS?CoV?2 核酸基因，重组酶介导等温核酸扩增技术（recombinase aided amplification，RAA）等新型常温核酸扩增技术也得到进一步应用。免疫学诊断包括胶体金和ELISA IgM 和IgG 抗体检测技术，主要用于人群的筛查和辅助诊断。基于CRISPR技术的新型诊断技术等也在进一步完善过程中。

Application of laboratory detection of Severe Acute Respiratory Syndrome Coronavirus-2

After the completion of the SARS-CoV-2 isolation, culture and genome sequencing, the diagnosis of new coronavirus pneumonia mainly depends on the detection of the SARS-CoV-2 nucleic acid by RT-PCR. In addition, the new room temperature nucleic acid amplification technologies such as RAA have been further applied in lab detection. Immunological diagnosis includes colloidal gold and IgM/IgG antibody detection technology, which is used for screening and auxiliary diagnosis of the population. New diagnostic technologies based on CRISPR are also in the process of further improvement.