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顺铂诱导SPC-A1人肺腺癌细胞凋亡过程中miR-27a的表达及意义
引用本文:朱善军,韩 月,徐 娟,谢而付,陈 丹,张美娟,张 燕,娄鉴芳,曹 艳,许雨乔,孙瑞红,王 芳,潘世扬.顺铂诱导SPC-A1人肺腺癌细胞凋亡过程中miR-27a的表达及意义[J].南京医科大学学报,2013(7):873-876.
作者姓名:朱善军  韩 月  徐 娟  谢而付  陈 丹  张美娟  张 燕  娄鉴芳  曹 艳  许雨乔  孙瑞红  王 芳  潘世扬
作者单位:南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029;南京医科大学第一附属医院检验学部,国家临床检验重点专科,江苏 南京 210029
基金项目:国家自然科学基金资助(81272324, 81000754, 81101322, 81201359);江苏省实验诊断学重点实验室基金(XK201114);江苏高校优势学科建设工程资助
摘    要:目的:检测miR-27a在顺铂(DDP)体外诱导人肺腺癌细胞株SPC-A1凋亡过程中的表达变化,探讨其与细胞凋亡之间的相关性及其检测的临床意义?方法:体外培养SPC-A1细胞,实验组加入终浓度为2.5 μg/ml DDP,同时设置不加DDP的对照组?观察12?24?48和72 h后显微镜下细胞形态学变化,流式细胞术检测细胞凋亡率,real-time PCR检测培养上清液中和SPC-A1细胞内miR-27a的表达变化?结果:DDP(2.5 μg/ml)组SPC-A1细胞形态呈凋亡改变,在48和72 h凋亡率分别为(59.3 ± 2.5)%和(76.4 ± 3.1)%,均显著高于对照组,差异有统计学意义(P < 0.05)?DDP (2.5 μg/ml)组培养上清液中和SPC-A1细胞内miR-27a含量在24?48和72 h均显著高于对照组,差异有统计学意义(P < 0.05)?DDP (2.5 μg/ml)组培养上清液中miR-27a含量在12?24?48和72 h逐渐升高,两两比较差异均有统计学意义(P < 0.05)?结论:miR-27a在DDP诱导的SPC-A1细胞凋亡中表达升高,提示其可能参与细胞凋亡调控;对miR-27a进行定量检测有望用于对肺腺癌化疗疗效的评估?

关 键 词:顺铂  肺癌SPC-A1  细胞凋亡  miR-27a
收稿时间:2/8/2013 12:00:00 AM

Expression changes and significance of miR-27a in cisplatin-induced apoptosis of human lung adenocarcinoma cell line SPC-A1
Zhu Shanjun,Han Yue,Xu Juan,Xie Erfu,Chen Dan,Zhang Meijuan,Zhang Yan,Lou Jianfang,Cao Yan,Xu Yuqiao,Sun Ruihong,Wang Fang and Pan Shiyang.Expression changes and significance of miR-27a in cisplatin-induced apoptosis of human lung adenocarcinoma cell line SPC-A1[J].Acta Universitatis Medicinalis Nanjing,2013(7):873-876.
Authors:Zhu Shanjun  Han Yue  Xu Juan  Xie Erfu  Chen Dan  Zhang Meijuan  Zhang Yan  Lou Jianfang  Cao Yan  Xu Yuqiao  Sun Ruihong  Wang Fang and Pan Shiyang
Institution:Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,National Key Clinical Department of Laboratory Medicine,Nanjing 210029,China
Abstract:
Keywords:cisplatin  SPC-A1  cell apoptosis  miR-27a
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