组织芯片技术检测MRP1/CD9在胃癌中的表达

目的:探讨运动相关蛋白-1(MRP1/CD9)在胃癌组织中的表达与胃癌生物学行为之间的相关性?方法:采用组织芯片技术及免疫组化方法对223例胃癌组织的MRP1/CD9蛋白表达进行研究?结果:在胃癌组织中MRP1/CD9表达的阳性率为58.3%,其表达与患者的性别?年龄?浸润深度?胃癌分期无关,而与肿瘤的分化程度?淋巴结转移密切相关?其中高-中分化组表达高于低分化组,差异有显著性(P < 0.05);无淋巴结转移组表达高于淋巴结转移组,亦具有显著性(P < 0.01)?二元Logistic回归前进法显示:淋巴结转移最先进入方程,是影响MRP1/CD9表达最显著的因素,其次是分化?结论:胃癌组织中MRP1/CD9表达改变与肿瘤的分化?淋巴结转移密切相关?

Expression of MRP1/CD9 in gastric cancer detected by tissue microarray

Objective:To investigate the correlation between the expression of MRP1/CD9 and the biological behaviors of gastric cancer. Methods:Tissue microarray and immunohistochemistry technique were used to detect the expression of MRP1/CD9 in 223 cases with gastric cancer. Results:The positive rate of MRP1/CD9 in gastric cancer patients was 58.3%,and the expression was not associated with gender,age,infiltration depth and stages,but had close relationship with the differentiated degree and lymphnode metastasis. The expression of MRP1/CD9 in moderate or high differentiated group was higher than that in the low differentiated group significantly (P < 0.05);which was the same when compared between non-lymphnode metastasis group and lymphnode metastasis group(P < 0.01). Forward stepwise binary logistic regression showed:lymphnode metastasis,which was first into the equation,is the most significant factor of MRP1/CD9 expression and followed by differentiation. Conclusion:The change of MRP1/CD9 expression in gastric cancer was closely correlated with the differentiated degree and lymphnode metastasis.