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三氧化二砷体外诱导人胃癌SGC-7901细胞凋亡的研究
引用本文:娄青林,冷静,彭韬,冯振卿.三氧化二砷体外诱导人胃癌SGC-7901细胞凋亡的研究[J].南京医科大学学报,2001,21(4):299-302.
作者姓名:娄青林  冷静  彭韬  冯振卿
作者单位:南京医科大学病理学教研室!江苏南京210029
基金项目:南京医科大学科技发展基金资助项目 (No 9662 )
摘    要:目的 :探讨三氧化二砷 (As2 O3 )对人胃癌SGC 790 1细胞抑制生长、诱导凋亡的生物学效应。方法 :光学显微镜观察细胞形态 ,四甲基偶氮唑蓝 (MTT)还原法检测As2 O3 对该细胞株生长的影响 ,流式细胞仪 (FCM )及 3′ OH末端标记法(TUNEL)检测凋亡细胞。结果 :SGC 790 1细胞经As2 O3 作用后 ,细胞增殖受到明显抑制 ,且呈剂量和作用时间依赖关系 ,细胞周期分析显示G2 M期阻滞 ;FCM检测在细胞周期G1期前出现低于 2倍体的凋亡峰 ;TUNEL标记发现DNA链的断裂。结论 :As2 O3 能抑制人胃癌SGC 790 1细胞增殖 ,并诱导该细胞系凋亡 ,其发生机制可能与细胞周期阻滞有关。

关 键 词:三氧化二砷  胃肿瘤  细胞凋亡  细胞周期阻滞
文章编号:1007-4368(2001)04-0299-04
修稿时间:2000年12月6日

Study on Arsenic Trioxide Induced Apoptosis of Human Gastric Carcinoma Cell Line SGC-7901 in Vitro
LOU Qing-lin,LENG Jing,PENG Tao,FENG Zhen-qing.Study on Arsenic Trioxide Induced Apoptosis of Human Gastric Carcinoma Cell Line SGC-7901 in Vitro[J].Acta Universitatis Medicinalis Nanjing,2001,21(4):299-302.
Authors:LOU Qing-lin  LENG Jing  PENG Tao  FENG Zhen-qing
Abstract:Objective:To study the biological effects of arsenic trioxide (As 2O 3) on human gastric carcinoma cell line SGC-7901 in vitro. Methods:By means of MTT reduction assay, the effect of As 2O 3 on the proliferation of SGC-7901 cells was measured. Morphological changes were observed under the light microscope. Ratio of apoptotic cell was detected by Flow Cytometry and TUNEL. Results:As 2O 3 could significantly inhibit the proliferation of SGC-7901 cells and the inhibitory effect was dose-and-time-dependent. As 2O 3 could also induce a G2/M phase arrest in this cell line. The cells treated with As 2O 3 showed a typical apoptotic morphology and hypodiploid peak before G1 phase. TUNEL detection analysis revealed the DNA fragmentation. Conclusion:As 2O 3 could induce apoptosis of SGC-7901 cells, which could be related to cell cycle arrest.
Keywords:arsenic trioxide  gastric neoplasm  apoptosis  cell cycle arrest
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