PGE2通过cAMP-PKA信号转导通路促进肝癌细胞VEGF表达

目的:探讨PGE2对人肝癌细胞HuH7VEGF表达的影响及其可能涉及的信号转导通路。方法:用PGE2、EP1-4四种受体激动剂、SQ22536+PGE2、H89+PGE2处理HuH7细胞,Real-time PCR、Western blot、ELISA等检测VEGFmRNA、蛋白表达的变化。结果:10μmol/L PGE2处理HuH7细胞24h后,VEGFmRNA的表达水平上升了264%(P<0.01)、细胞培养上清中VEGF蛋白表达水平上升了24%(P<0.01);10μmol/L PGE2处理HuH7细胞6、12、24h后,胞浆中VEGF蛋白的表达水平分别上升了13.6%、25.7%、39.6%(P<0.01);10μmol/L EP2受体激动剂处理HuH7细胞24h后,胞浆、细胞培养上清中VEGF蛋白表达水平与对照组相比分别上升了32.3%、20.1%(P<0.01),而10μmol/L EP1、EP3、EP4受体激动剂处理HuH7细胞24h后,VEGF的表达水平无明显改变;30μmol/L PKA抑制剂(H89)、500μmol/L腺苷酸环化酶抑制剂(SQ22536)处理HuH7细胞24h后,胞浆VE...

Prostaglandin E2 increased the expression of vascular endothelial growth factor (VEGF) by activation of cAMP-PKA pathway in hepatocellular carcinoma cells

Objective:To explore VEGF expression induced by PGE2 in human hepatocellular carcinoma cells HuH7 and the possible mechanisms of signal transduction pathways. Methods:HuH7 cells were treated with PGE2,EP1-4 receptor agonist,SQ22536+PGE2, H89+PGE2. VEGF mRNA and protein expression were examined by Real-time PCR,Western blot and ELISA in HuH7 cells. Results: The expression of VEGF mRNA in HuH7 cells were increased by 264%(P < 0.01),and the expression of VEGF in culture supernatant of HuH7 cells were increased...