| 兔视网膜色素上皮细胞培养方法的改进 |
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| 引用本文： | 张爱霞,王斌,肖继皋.兔视网膜色素上皮细胞培养方法的改进[J].南京医科大学学报,2003,23(2):146-147,190,F003. |
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| 作者姓名： | 张爱霞 王斌 肖继皋 |
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| 作者单位： | 南京医科大学药理教研室，南京医科大学药理教研室，南京医科大学药理教研室 江苏南京 210029，江苏南京 210029，江苏南京 210029 |
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| 基金项目： | 教育部留学回国人员科研启动基金资助项目(2002-01)，南京医科大学创新基金资助项目(CX2001003) |
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| 摘 要： | 目的：寻求一种更好的培养兔视网膜色素上皮(RPE)细胞的方法。方法：在无菌滤纸上剪开眼球，固定眼杯，用胰酶直接消化视网膜色素上皮面，收取RPE细胞。将初次换液时间由取材后48h延长至72h，记录改变换液时间对细胞数量及细胞形态等的影响。用多巴(DOPA)染色和免疫组织化学对细胞鉴定。结果：采用改进后方法收取的细胞呈分散均匀生长。延长初次换液时间为72h后获得的细胞增多，原代细胞长至融合状态需要的时间缩短，色素颗粒脱失现象减轻。结论：采用该方法更适于RPE细胞的收获和培养。
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| 关 键 词： | 视网膜色素上皮细胞 细胞培养 视网膜血管新生 胰酶消化法 |
| 文章编号： | 1007-4368(2003)02-0146-03 |
Improvement of Culture of Retinal Pigment Epithelium Cells in Rabbits |
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| ZHANG Ai-xia,WANG Bin,XIAO Ji-gao.Improvement of Culture of Retinal Pigment Epithelium Cells in Rabbits[J].Acta Universitatis Medicinalis Nanjing,2003,23(2):146-147,190,F003. |
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| Authors: | ZHANG Ai-xia WANG Bin XIAO Ji-gao |
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| Abstract: | Objective: To improve the cultivating method of retinal pigment epithelium cells (RPE) in rabbits. Methods:The eye ball was bisected along the equator on a sterile filter paper. The anterior portion of eye was discarded, then the posterior eye cup was fixed in a rubber bysma. The single cell was collected directly by trypsinization. The first time for the medium to be changed was prolonged from 48 h to 72 h. The RPE cells were identified by DOPA staining and immunohistochemistry. Results: RPE cells grew in well-distributed way rather than in colony in this experimental condition. Prolonging first changing medium time was found to increase the number of cells. RPE cells reached confluence more quickly and the melanin disappeared more slowly. Conclusion: The modified method is more suitable for cultivating RPE cells in rabbits. |
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| Keywords: | retinal pigment epithelium cell culture methods |
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