首页 | 本学科首页   官方微博 | 高级检索  
检索        

氯沙坦对高糖培养的人系膜细胞血管内皮细胞生长因子表达的影响
引用本文:汪颖南,邓红,苏宁,赵龙,刘必成,刘东风.氯沙坦对高糖培养的人系膜细胞血管内皮细胞生长因子表达的影响[J].南京医科大学学报,2005,25(3):150-153.
作者姓名:汪颖南  邓红  苏宁  赵龙  刘必成  刘东风
作者单位:东南大学病理与病理生理学系 江苏南京210009 (汪颖南,邓红,苏宁,赵龙),东南大学附属中大医院肾内科 江苏南京210009 (刘必成),东南大学病理与病理生理学系 江苏南京210009(刘东风)
基金项目:国家自然科学基金资助项目(30370658)
摘    要:目的:探讨氯沙坦对高糖培养的系膜细胞产生活性氧和血管内皮细胞生长因子(VEGF)mRNA表达的影响。方法:体外培养人系膜细胞,用高糖、氯沙坦干预不同时间后,应用比色法检测培养细胞上清液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活力,丙二醛(MDA)的水平。用RT鄄PCR法测定血管内皮细胞生长因子(VEGF)mRNA表达的影响。结果:与正常对照组相比,高糖组SOD、CAT的活力明显降低,而MDA含量明显升高,VEGFmRNA表达升高。与高糖组相比,高糖加氯沙坦组的SOD、CAT的活力升高,MDA水平下降,VEGFmRNA表达上调。结论:氯沙坦能抑制高糖所致细胞活性氧的产生和VEGF的表达,从而抑制细胞通透性升高,降低蛋白尿,发挥其肾保护作用。

关 键 词:氯沙坦  系膜细胞  活性氧  血管内皮细胞生长因子
文章编号:1007-4368(2005)03-0150-04
修稿时间:2004年4月1日

Effect of losartan on expression of human endothelial vascular growth factor Mrna of cultured human mesangial cells in high glucose
WANG Ying-nan,DENG Hong,SU Ning,ZHAO Long,LIU Bi-cheng,LIU Dong-feng.Effect of losartan on expression of human endothelial vascular growth factor Mrna of cultured human mesangial cells in high glucose[J].Acta Universitatis Medicinalis Nanjing,2005,25(3):150-153.
Authors:WANG Ying-nan  DENG Hong  SU Ning  ZHAO Long  LIU Bi-cheng  LIU Dong-feng
Institution:WANG Ying-nan*,DENG Hong,SU Ning,ZHAO Long,LIU Bi-cheng1,LIU Dong-feng
Abstract:Objective: To investigate the effect of Losartan on the production of reactive oxygen species(ROS) and the expression of endothelial vascular growth factor(VEGF) in human mesangial cells cultured in high glucose. Methods:Human mesangial cells were cultured in high glucose with or without Losartan, the activities of SOD and CAT, the level of MDA in the supernatant were measured by spectrophotometry and the expression of VEGF mRNA in the cells were measured by RT-PCR. Results:Compared with the control group, the activities of SOD and CAT were markedly lower, while the level of MDA was greatly higher in the high glucose group. High glucose stimulated the expression of VEGF mRNA. Compared with the high glucose group, high glucose with Losartan heightened the activities of SOD and CAT, declined the level of MDA, and down-regulated the expression of VEGF mRNA. Conclusion:Losartan might inhibit the production of ROS and down-regulate the expression of VEGF mRNA induced by high glucose. Thus the celluar permeability was suppressed, and the proteinuria was reduced. This maybe another mechanism which protects renal function from diabetic nephropathy .
Keywords:Losartan  mesangial cell  reactive oxygen species  vascular endothelial growth factor  
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《南京医科大学学报》浏览原始摘要信息
点击此处可从《南京医科大学学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号