PGE2通过EP2受体激活cAMP-PKA-CREB信号通路上调SnoN的表达而促进CCLP1细胞的增殖

目的:探讨前列腺素E2(prostaglandin E2,PGE2)对人胆管上皮癌细胞CCLP1增殖能力影响的作用机制。方法:用PGE2、EP1～4 4种受体激动剂、AC激动剂Forskolin、PKA抑制剂H89、cAMP拟似物db-cAMP处理CCLP1细胞,通过RT-PCR、Western blot、WST等实验检测SnoN mRNA、SnoN蛋白等表达水平、CREB蛋白磷酸化水平以及CCLP1细胞增殖能力的变化。结果:10μmol/L PGE2处理CCLP1细胞24 h后,SnoN mRNA的表达水平与对照组相比上升了22.5%(P<0.01),SnoN蛋白的表达水平上升了35.6%(P<0.05);10μmol/L EP受体激动剂处理CCLP1细胞24 h后,SnoN蛋白的表达水平与对照组相比明显增高,其中以EP2受体激动剂的作用最明显,上升了64.9%(P<0.05),细胞增殖能力上升了26.5%;10μmol/L AC激动剂Forskolin处理CCLP1细胞24 h后,SnoN蛋白的表达水平及CREB蛋白的磷酸化水平较对照组分别上升了25.1%、71.3%(P<0.05),细胞增殖能力也上升了...

Prostaglandin E2 promotes the cell prolifelation ability of CCLP1 through EP2 prostanoid receptor which increased the expression of SnoN by activation of cAMP-PKA-CREB pathway

Objective: To explore the mechanism of prostaglandin E2(PGE2)affects the cell proliferation ability of human bile duct carcinoma cells CCLP1.Methods:CCLP1 cells were treated with PGE2,EP1-4 receptor agonist,AC agonist(Forskolin),PKA antagonist(H89),cAMP analogues(db-cAMP).The expression levels of SnoN mRNA and SnoN protein,and the cell proliferation ability were examined by RT-PCR,Western blot and WST methods in CCLP1 cells.Results:The expression of SnoN mRNA in CCLP1 cells increased by 22.5%(P < 0.01),whil...