Rab基因家族在MDA-MB-231乳腺癌细胞胞体和伪足中的差异表达

目的:以表皮生长因子(epidermal growth factor,EGF)刺激MDA-MB-231乳腺癌细胞胞体和伪足分离,在此基础上,鉴定Rab基因家族在胞体和伪足中的差异表达谱?方法:运用Boyden小室技术分离EGF刺激下MDA-MB-231乳腺癌细胞的胞体和伪足,并鉴定Rab基因家族在乳腺癌细胞胞体和伪足中的差异表达谱?结果:RAB1A等21个基因在胞体中高表达,RAB7A等16个基因在伪足中高表达,RAB3B等24个基因在胞体和伪足中的表达量大体相等?结论:本研究建立的基于Boyden小室技术的实验方法可以有效分离乳腺癌细胞的胞体和伪足,在此基础上鉴定了一类在EGF趋化刺激下乳腺癌细胞伪足中富集的Rab 基因,从而为进一步深入研究乳腺癌细胞伪足伸展的确切分子机制提供新的思路和研究方向?

The differential expression of Rab gene family in cell bodies and pseudopodia in MDA-MB-231 breast cancer cells

Objective:To establish Boyden chamber approaches for comparative analysis of the cell body and pseudopodium protein or RNA of chemotactic breast cancer cells. Methods:Under EGF stimulation,the cell body and pseudopodium protein or RNA of MDA-MB-231 breast cancer cells were separated using Boyden chamber approaches. Then the differential expression of Rab gene family in cell bodies and pseudopodia of MDA-MB-231 breast cancer cells were analyzed. Results:Protein or RNA of cell bodies and pseudopodia were successfully separated and used to analyze the differential expression of Rab gene family in MDA-MB-231 breast cancer cells. We found that 21 Rab genes were highly expressed in cell bodies,16 Rab genes were highly expressed in pseudopodia,and the other 24 Rab genes were equally expressed in cell bodies and pseudopodia. Conclusion:These results make advances in our understanding of the molecules that abundantly located in breast cancer cells pseudopodia.