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Induction of interleukin-8 production by angiotensin Ⅱ in rat vascular smooth muscle cells
作者姓名:Zhi Wang  Lili Zhang  Baogui Sun  Qiuyan Dai
作者单位:Department of Cardiology,the First People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200080,China  
基金项目:supported by the Shanghai Science and Technology Committee Grant (No.03DJ14019)
摘    要:Objective:Interleukin-8(IL-8) represents the prototypical chemokine that is made by a wide variety of cell types.Previously studies have suggested that angiotensin Ⅱ(Ang Ⅱ) is involved in atherogenesis through induction ofproinflammatory cytokines such as interleukin-6 or monocyte chemoattractant protein-1 (MCP-1) in vascular smooth muscle cells(VSMCs),while the role orang Ⅱ on IL-8 expression in VSMCs is poorly studied.Methods:In this study,VSMCs were isolated from the thoracic aorta of Sprague-Dawley rats.The expression of smooth muscle α-actin was confirmed by an immunohistochemical method.Semi-quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) analyses were conducted to detect IL-8 expression.Results:In the present study we found that Ang Ⅱ significantly increased the expression of IL-8 both at the mRNA and protein levels in rat VSMCs in a dose- and time-dependent manner.Conclusion:These findings suggested that Ang Ⅱ may participate in atherosclerosis through induction of inflammatory mediator in VSMCs.

关 键 词:Angiotensin    Interleukin-8  Vascular  smooth  muscle  cells  
收稿时间:13 October 2008

Induction of interleukin-8 production by angiotensin II in rat vascular smooth muscle cells
Zhi Wang,Lili Zhang,Baogui Sun,Qiuyan Dai.Induction of interleukin-8 production by angiotensin II in rat vascular smooth muscle cells[J].Acta Universitatis Medicinalis Nanjing,2009,29(1):50-53.
Authors:Zhi Wang  Lili Zhang  Baogui Sun and Qiuyan Dai
Institution:Department of Cardiology, the First People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, China
Abstract:

Objective

Interleukin-8(IL-8) represents the prototypical chemokine that is made by a wide variety of cell types. Previously studies have suggested that angiotensin II(Ang II) is involved in atherogenesis through induction of proinflammatory cytokines such as interleukin-6 or monocyte chemoattractant protein-1(MCP-1) in vascular smooth muscle cells(VSMCs), while the role of Ang II on IL-8 expression in VSMCs is poorly studied.

Methods

In this study, VSMCs were isolated from the thoracic aorta of Sprague-Dawley rats. The expression of smooth muscle α-actin was confirmed by an immunohistochemical method. Semi-quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) analyses were conducted to detect IL-8 expression.

Results

In the present study we found that Ang II significantly increased the expression of IL-8 both at the mRNA and protein levels in rat VSMCs in a dose- and time-dependent manner.

Conclusion

These findings suggested that Ang II may participate in atherosclerosis through induction of inflammatory mediator in VSMCs.
Keywords:Angiotensin II  Interleukin-8  Vascular smooth muscle cells
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