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ERK1/2和Survivin在食管癌中的表达及其相关性
引用本文:冯亚东,郝 波,朱 宏,施瑞华.ERK1/2和Survivin在食管癌中的表达及其相关性[J].南京医科大学学报,2009,29(8):1085.
作者姓名:冯亚东  郝 波  朱 宏  施瑞华
作者单位:南京医科大学第一附属医院消化科,江苏,南京,210029  
基金项目:江苏省科技厅基础研究计划 
摘    要:目的:探讨ERK1/2和Survivin在食管癌中的表达和二者之间的相关性.方法:应用免疫组化法检测4JD例食管癌和20例正常食管标本中ERK1/2和Survivin的表达.Western blot法检测ERK1/2和Survivin在食管癌中的表达以及PD98059对食管癌Ecal09细胞株ERK1/2和Survivin表达的影响.免疫共沉淀法检测ERK1/2和Survivin在食管癌Eca109细胞株中的相互作用.结果:ERK1/2和Survivin在食管癌中表达的阳性率分别87.5%和90.0%.在正常食管组织中表达的阳性率为40.0%和5.0%.Westen blot显示ERK1/2和Survivin在食管癌癌组织中的表达高于癌旁组织中的表达.免疫共沉淀结果提示ERK1/2和Survivin在食管癌中的表达存在相互作用.PD98059下调Survivin的表达.结论:ERK1/2和Survivin在食管癌中高表达.在食管癌Ecal09细胞中ERK1/2和Survivin表达有相关性.抑制ERK1/2的表达能下调Survivin的表达.在食管癌中Survivin的表达可能依赖于ERK1/2.

关 键 词:食管癌  相关性
收稿时间:2009/3/31 0:00:00

The expression and correlation of ERK1/2 and Survivin in esophageal cancer
FENG Ya-dong,HAO Bo,ZHU Hong and SHI Rui-hua.The expression and correlation of ERK1/2 and Survivin in esophageal cancer[J].Acta Universitatis Medicinalis Nanjing,2009,29(8):1085.
Authors:FENG Ya-dong  HAO Bo  ZHU Hong and SHI Rui-hua
Institution:Department of Gastroenterology;the First Affiliated Hospital of NJMU;Nanjing 210029;China
Abstract:Objective:To investigate the expression and correlation of ERK1 /2 and Survivin in esophageal cancer. Methods:The expression of ERK1/2 and Survivin on 40 esophageal cancer tissues and 20 normal esophageal tissues were detected by immunohis-tochemistry. The protein expression of ERK1/2 and Survivin and the consequent changes by PD98059 were detected by Western blot analysis. The correlation of ERK1/2 and Survivin in Eca109 cells was detected by co-immunoprecipitation analysis. Results: Among 40 esophageal ca...
Keywords:ERK1/2  Survivin
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