紫草素对人胎肾小球系膜细胞增殖、凋亡及细胞外基质的影响

目的：研究紫草素对人胎肾小球系膜细胞(MC)增殖、凋亡及细胞外基质的影响。方法：采用人胎肾细胞培养法、MTT法、流式细胞仪及免疫组化的方法。结果：紫草素0．05、0．10、0．50及1，00mmol／L浓度对培养24、48、72h人MC有明显抑制作用；紫草素明显抑制高糖诱发的人MC细胞凋亡；紫草素0．05、0．10、0．50μg/ml显著抑制系膜基质层粘连蛋白(LN)、纤维连接蛋白(FN)及胶原Ⅳ(CoⅣ)的生成。结论：紫草素具抑制人MC增殖、细胞外基质生成及细胞凋亡作用。

Effects of Shikonin on Proliferation, Apoptosis, and Extracellular Matrix of Human Mesangial Cells

Objective: To investigate the effect of shikonin on the proliferation, apoptosis and extracellular matrix (ECM) of human mesangial cells (MC). Methods : MC was cultured in vitro with different concentrations of glucose (30, 50, 80 mmol/L). The cell growth was observed by using MTT method and apoptosis by using an aunexin-V-Fluos.Immunohistochemical studies for Laminin (LN), Fibronectin (FN) and type Ⅳ Collagens (Col Ⅳ ) were measured. Results: Shikonin inhibited their growth ( P ＜ 0.05) and apoptosis in the glycated cultured cells. Shikonin 0.05 mmol/L significantly reduced the secretion of LN, FN and Col Ⅳ from MC (P ＜ 0.05) cultured in 30, 50 and 80 mmol/L glucose.Conclusion: Shikonin could prevent or treat diabetic nephropathy (DN) and glomerulosclerosis (GS).