不同起源胶质瘤中microRNA-210表达差异及其与星形胶质细胞肿瘤级别的相关性

目的:探讨microRNA-210(miR-210)在星形胶质细胞肿瘤和少突胶质细胞肿瘤中的表达差异及意义?方法:将组织标本分为正常脑组织?少突胶质细胞肿瘤?星形胶质细胞肿瘤3组,星形胶质细胞肿瘤再分为A毛细胞型星形细胞瘤(WHO Ⅰ级)]?B弥漫性星形细胞瘤(WHO Ⅱ级)]?C间变性星形细胞瘤(WHO Ⅲ级)]?D胶质母细胞瘤(GBM,WHO Ⅳ级)]4个亚组?采用实时定量PCR方法检测各组中miR-210的表达水平?统计学分析miR-210的表达和星形胶质细胞肿瘤级别的相关性?结果:miR-210在各级别星形胶质细胞肿瘤中的表达水平依次为D> C> A和B,除A?B组间外,其他组间的差异都具有统计学意义(P < 0.001),星形胶质细胞肿瘤级别越高,miR-210表达水平越高;然而miR-210在少突胶质细胞肿瘤中呈低表达(P < 0.05),且间变性少突胶质细胞瘤中表达量低于少突胶质细胞瘤中的表达量?结论:miR-210在不同起源?不同级别胶质瘤中的表达水平不同,可以作为不同起源胶质瘤病理检查的辅助鉴别手段,也可作为星形胶质细胞肿瘤恶性进展的生物学标志物?

Correlation between microRNA-210 expression and grades of astrocytoma

Objective:To investigate the significance and different expressions of microRNA-210 in astrocytic tumors and oligodendroglial tumors. Methods:Tissue samples were divided into three groups including the normal brain tissue group,the oligodendroglial tumor group and the astrocytic tumor group. The astrocytic tumors were divided into four groups including group Apilocytic astrocytoma (WHO grade I)],Bdiffuse astrocytomas (WHO grade II)],Canaplastic astrocytomas(WHO grade III)]and Dglioblastoma (GBM,WHO grade IV)]. The expression of miR-210 was examined by real-time quantitative RT-PCR. The correlation between the expression of miR-210 and the astrocytic tumors was analyzed by Spearman correlation test. Results:The expression level order of miR-210 was group D> group C> group B and group A. There was statistically significant difference between groups except group A and B (P < 0.001). The grade of astrocytic tumor and the expression of miR-210 had positive correlation. However,the expression level of miR-210 in anaplastic astrocytomas was lower than in oligodendroglial tumor,and was low in oligodendroglial tumor (P < 0.005). Conclusion:Since expression of miR-210 showed significant differences in different origins and different grades,it can be used as an auxiliary identification method for the histopathologic examination of glioma from different origins,or as a biomarker for the observation of the progression of astrocytic tumor as well.