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Molecular cloning of human heat shock protein 27 and study of its protective effects on oxidative damage in rat cardiomyocte H9c2
作者姓名:Li Liu  Xiaojin Zhang  Surong Jiang  Xiang Gao  Guoxain Ding  Yunlin Cheng
作者单位:Li Liu~1,Xiaojin Zhang~2,Surong Jiang~1,Xiang Gao~1,Guoxain Ding~1 and Yunlin Cheng~1 ~1Department of Gerontology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China;~2Center of Model Animal,Nanjing University,Nanjing 210045,China.
基金项目:Chuangxin Funding of Nanjing Medical University (CX2003018)
摘    要:INTRODUCTIONApgionsgt-imnit omtiuclt icceellllsu slaurch o ragsa nciasrmdiso imsy omcoysttes e vaindde nnte iurons1].Cardiomyocytes aging is associated with an increasedincidence of cardiac arrhythmias and diastolic ansystolic dysfunction,which may ultimately lead to hearfailure.Because of enormous suffering and health-carburdenthat cardiac dysfunction causes,much effort hagoneinto both understanding the mechanisms leading tage-related myocardial decline and discovering the accesses to re…

关 键 词:分子系化  人类热休克蛋白27  保护机制  氧化损伤  H9c2  心肌细胞
收稿时间:2004-01-17

Molecular cloning of human heat shock protein 27 and study of its protective effects on oxidative damage in rat cardiomyocte H9c2
Li Liu,Xiaojin Zhang,Surong Jiang,Xiang Gao,Guoxain Ding,Yunlin Cheng.Molecular cloning of human heat shock protein 27 and study of its protective effects on oxidative damage in rat cardiomyocte H9c2[J].Acta Universitatis Medicinalis Nanjing,2005,19(4):187-190.
Authors:Li Liu  Xiaojin Zhang  Surong Jiang  Xiang Gao  Guoxain Ding  Yunlin Cheng
Institution:1. Department of Gerontology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
2. Center of Model Animal, Nanjing University, Nanjing 210045,China
Abstract:Objective: To clone human cardiac heat shock protein 27(HSP27) gene and to determine the effects of HSP27 on the oxidative stress in rat cardiomyocyte cell line H9c2. Methods: Full length of HSP27 cDNA which got by RT-PCR was constructed into pCDNA3.1 + . The recombinant was transfected into rat cardiomyocyte cell line H9c2 and the stable transfection cell line was selected by G418. Then we observe the effects of HSP27 over-expression on LDH release and apoptosis induced H2O2 in H9c2. Results:1000 μmol/L H2O2 in HSP27 over-expression group and wild type group were 0.396 ± 0.017 vs. 0.390 ± 0.009 ( p > 0.05 ), 0.437 ± 0.014 vs. 0.416 ± 0.015 ( P < 0.05), 0.471 ± 0.018 vs. 0.417 ± 0.009 ( P < 0.001), 0. 505 ± 0.030 vs. 0. 657 ± 0.022 ( P < 0.001),and wild type group were (10.693 ± 1.122) % vs. (4.027 ± 1.628 )% ( P < 0.01). Conclusion: We cloned and constructed human cardiac HSP27 gene successfully, and over-expression of human HSP27 could inhibit oxidative damage significantly in H9c2.
Keywords:Heat Shock Protein 27  Molecular Cloning  Transfection  Oxidafive Stress  Apoptosis
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