| 心肌急性损伤肌球蛋白轻链激酶酶促反应动力学研究 |
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| 引用本文： | 黄峻 陈庆. 心肌急性损伤肌球蛋白轻链激酶酶促反应动力学研究[J]. 南京医科大学学报(自然科学版), 1995, 15(3): 506-511 |
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| 作者姓名： | 黄峻 陈庆 |
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| 作者单位： | 第一附属医院心脏科 |
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| 摘 要： | 以内毒素休克狗作为急性心肌损害的动物模型，从心肌中提纯肌球蛋白轻链（ＭＬＣ）和肌球蛋白轻链激酶（ＭＬＣＫ），根据酶动力学分析法研究急性损害对ＭＬＣＫ活性的影响。结果发现在此种状态下狗心肌ＭＬＣＫ活性显著降低，推测ＭＬＣＫ活性降低导致可磷酸化轻链（ＰＬＣ）磷酸化的磷酸酶相对占优势，磷酸化型Ｐ－ＬＣ向去磷酸化型转变。
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| 关 键 词： | 心肌损伤 肌球蛋白 肌球蛋白轻链 狗 MLCK |
The Kinetics of Myosin Light Chain Kinase from Canine Cardiac Muscle with Acute Myocardial Injury |
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| Huang Jun,Chen Qing,Kong Qiangqing,et al.. The Kinetics of Myosin Light Chain Kinase from Canine Cardiac Muscle with Acute Myocardial Injury[J]. Acta Universitatis Medicinalis Nanjing, 1995, 15(3): 506-511 |
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| Authors: | Huang Jun Chen Qing Kong Qiangqing et al. |
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| Abstract: | In this study we isolated myosin light chain(MLC)and myosin light chain kinase(MLCK)from acute myocardial injury model of dog caused by endotoxin shock. The kinetic change of MLCK during endotoxin shock was analysed. Results showed that MLCK activity in canine cardiac muscle with endotoxin shock was markedly reduced. It is reasonable to think that the decrease of the MLCK activity resulted in the phosphatase being predominant under this condition,with a shift of phosphorylated type of MLC light chain(P-LC) to dephosphorylated type. |
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| Keywords: | cardiac muscle myosin myosin light chain myosin light chain kinase dogs |
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