色素上皮衍生因子抑制脉络膜新生血管的实验研究

目的：观察重组人色素上皮衍生因子（recombinant human Pigment Epithelium—Derired Factor．rhPEDF）对氩激光诱导的棕色挪威（brown Norway，BN）大鼠脉络膜新生血管的抑制作用。方法：对2组10只BN大鼠单眼行氩激光视网膜光凝，建立脉络膜新生血管模型。光凝后1周，一组动物玻璃体内注射2ug rhPEDF／10uL PBS；另一组动物玻璃体内注射10uL的PBS作为对照组。光凝后2周，对2组动物进行眼底荧光血管造影（fundus fluorescence vascular imaging，FFA）、组织病理学检查及光镜下测量脉络膜新生血管（choroidal neovasculanzation，CNV）厚度并对FFA结果及CNV厚度进行图像及统计学分析，以评估rhPEDF对脉络膜新生血管的抑制效果。结果：视网膜光凝后2周，FFA检查对照组与rhPEDF组相比，荧光素渗漏明显增加，差异有统计学意义（P〈0．05）；CNV厚度检测结果，rhPEDF组光凝区CNV厚度低于对照组，差异有统计学意义（P〈0．05）。结论：对氩激光诱导形成CNV的BN大鼠玻璃体腔内注射rhPEDF，可以抑制CNV的增殖。

Experimental Study on the Inhibition of PEDF on CNV

Objective: To observe the inhibition of rhPEDF on CNV of BN rats induced by argon laser.Methods: 10 BN rats divided into two groups were photocoagulated by argon laser in single eye to set up the CNV model.1 week later,one group of 5 rats were received intravitreal injections of 2μg rhPEDF/10μl PBS,and the other group of 5 rats were received intravitreal injections of 10μl PBS as control group.2 weeks later,FFA and histopathologic examination were performed in two groups and the thickness of laser-induced CNV was measured by light microscope.The results of the FFA and CNV thickness were statistically analyzed to assess the inhibitory effect of rhPEDF on CNV.Results:2 weeks later after photocoagulation,FFA showed less fluorescein leakage in the rhPEDF group than that in the control group.There was statistical difference between the two groups(P<0.05).The thickness of CNV in rhPEDF group was less than that in the control group.There was statistical difference between the two groups(P<0.05).Conclusion: Intravitreal rhPEDF injection can inhibit CNV induced by argon laser in BN rat.