地塞米松对大鼠肺泡巨噬细胞吞噬功能的影响

目的：通过研究地塞米松对大鼠肺泡巨噬细胞吞噬功能的影响，探讨地塞米松治疗急性肺损伤的作用机理。方法：健康雄性Wistar大鼠放血处死后全肺灌洗获取肺泡巨噬细胞（PAMs），贴壁纯化，在预培养20h后重悬PAMs，分3组分别处理4h。正常对照（C）组：不做任何干预；脂多糖（LPS）组：按10μg／ml。的剂量加入LPS刺激；脂多糖加地塞米松（LPS＋Dex）组：将含Dex终浓度1×10mmol／L～1×6mmol／L的RPMI 1640培养基加入LPS。收集PAMs，与1％鸡红细胞悬液培育后制片，瑞氏染色观察并计算吞噬率和吞噬指数，对两者做相关回归分析并采用单因素方差分析比较组间差异。结果：各组AM的吞噬率和吞噬指数呈线性相关性关系．从高到低依次为：C组（12．50±2．07）％，2．29±0．08；LPS＋DeX组（8．25±1．67）％，1．73±0．18；LPS组（3．50±1．20）％，1．16±0．19；各组间的差异均具有统计学意义（P〈0．05）。结论：LPS可抑制体外培养PAMs的吞噬活性；Dex对LPS抑制的PAMS吞噬活性有一定的活化作用。

Effect of Dexamethasone on Phagocytic Function of Rat Pulmonary Alveolar Macrophages

Objective： To investigate the mechanism of dexamethasone in treatment of acute lung injury by studying the effect of dexamethasone on phagocytic function of rat alveolar macrophages. Methods： After the healthy male Wistar rats were sacrificed to carry out whole lung lavage so as to take pulmonary alveolar macrophages and perform adherent purification, PAMs were resuspended after 20 h pre-culture and divided into 3 groups for intervention treatment for 4 hours respectively. The normal control（C） group was not interventioned. Lipopolysaccharide （LPS）group was stimulated by 10 μg/mL and LPS. LPS and Dex （LPS ＋ Dex） group was treated by contained Dex final concentration 1 - 10 mmol/L - 1×6 mmol/L RPMI 1 640 medium and LPS. PAMs were collected and incubated with 1% chicken eryth-rocyte suspension to make slice. Wright＇s staining was implemented for calculating the ratio and index of phagocytosis. The two targets were treated with correlation-regression analysis and the disparity of 3 groups were compared with one- factor analysis of variance. Results： The phagocytic ratio and index of AM in each group showed linear correlation . The ratio and index of phagocytosis from descending order of the 3 groups were （ 12.50 ±2.07） % ,2.29±0.08 in group C, （8.25±1.67）% ,1.73±0.18 in group LPS and Dex and （3.50±1.20）% ,1.16±0.19 in group LPS respectively. There were statistically significant differences between the 3 groups （P 〈 0.05）. Conclusion： LPS could inhibit the phagocytic activity of PAMs cuhured in vitro. Dex has some activation for the PAMs phagocytic activity inhibited by LPS.