噻萘普汀对慢性应激大鼠海马体积影响的在体研究

目的 在体观察噻萘普汀对强迫游泳大鼠海马体积的影响.方法 将24只大鼠随机分成4组:对照组(n=6)、4周应激组(n=6)、4周应激生理盐水组(n=6)和4周应激噻萘普汀组(n=6),各应激组大鼠接受连续4周的强迫游泳应激.根据行为学和血清皮质酮水平评定应激效果,4周后行MRI扫描在体检测左右海马的体积.结果 4周应激组、4周应激加水组及4周应激给药组的血清皮质酮水平(728.67±77.67)ng/ml,(765.37±250.87)ng/ml,(863.49±282.31)ng/ml],均比对照组(191.60±116.41)ng/ml]明显升高(P＜0.01).左、右侧绝对海马体积比较,4周应激组(59.45±5.05)mm3,(61.66±5.32)mm3]和4周应激加水组(61.01±6.06)ng/ml,(61.43±3.12)ng/ml]均比对照组明显减小(P＜0.01);4周应激给药组大鼠均较4周应激组和4周应激加水组明显增加(P＜0.01),而与对照组之间差异无显著性(P＞0.05).大鼠双侧海马相对体积组间比较结果与绝对体积一致.应激后大鼠爬格数与海马体积呈正相关(r=0.557～0.598,P＜0.05).结论 噻萘普汀可逆转强迫游泳慢性应激导致的大鼠双侧海马体积减少.

In vivo study of effect of tianeptine on hippocampal volume of chronic stressed rats

Objective To observe the change of the hippocampal volume of rats induced by chronic stress and effects of tianeptine on it. Methods 24 rats were randomly allocated into control(n=6), 4 week-stress(n=6), 4 week-stress with saline(n=6)and 4 week-stress with tianeptine(n=6)groups. Forced Swimming Test was used to set up stress animal model. the stressful responses were assessed by behaviour and serum corticosterone level. and hippocampal volume was measured in vivo by MRI. Results The serum corticosterone level of all the stressed groups(728. 67±77. 67, 765. 37±250. 87, 863. 49±282. 31)are significantly higher than that of control group(191. 60 ±116. 41), P＜0. 01. In absolutely left and right hippocampal volume, 4-week stress group(59. 45±5. 05, 61. 66±5. 32)and 4-week stress with saline group(61. 01±6. 06, 61. 43±3. 12)have smaller hippocampal volumes compared with control group(all P＜0. 01);4-week stress group with tianeptine has much bigger bilateral hippocampal volume than the 4-week stress group and 4-week stress with saline group(all P＜0. 01), but there is no significant difference between 4-week stress group with tianeptine and control group. The comparison of adjusted bilateral hippocampal volume is consistent with the absolute hippocampal volume. In addition, there is a positive correlation between the amount of acrossing and hippocampal volume, the coefficient correlations range from 0. 0557～0. 0598(P＜0. 05). Conclusion It was indicated that tianeptine could reverse the reduction of hippocampal volume caused by chronic stress.