结直肠癌中差异性表达的外泌体lncRNA功能富集及通路分析

  目的  通过探讨差异性表达的外泌体lncRNA及其调控的信号通路在结直肠癌发生、发展中的作用，以期为结直肠癌的诊断和治疗提供新的靶点。  方法  使用R软件线性回归模型软件包limma包进行差异表达分析，根据筛选阈值得到显著差异表达的lncRNA和mRNA；通过计算差异表达lncRNA与mRNA皮尔逊相关性系数，获得其之间协同表达关系，通过相关系数筛选存在正向协同表达的lncRNA-mRNA关系对；分别对lncRNA的协同表达mRNA进行GO功能和KEGG通路富集分析，以间接预测lncRNA的功能。  结果  在32例健康志愿者和12例结直肠癌患者中筛选出差异性表达的外泌体lncRNA共202个、mRNA共1 014个。验证出差异性表达的lncRNA-mRNA关系对10个。对lncRNA协同表达的mRNA进行功能富集显示，差异性表达的外泌体lncRNA可能与B细胞受体信号通路、NF-κB信号通路、JAK-STAT信号通路、抗原处理与呈递信号通路和NOD样受体信号通路活化密切相关。  结论  深入探索外泌体lncRNA在结直肠癌中的差异性表达、功能富集及通路活化，有助于为结直肠癌的治疗提供新思路，为诊断提供新靶点。 

Functional enrichment and pathway analysis of differential expression of exosome lncRNA in colorectal cancer

Objective To explore the role of differential expression of exosome lncRNA and its regulated signal pathway in the occurrence and development of colorectal cancer and provide a new target for the diagnosis and treatment of colorectal cancer.Methods The linear regression model package limma of R software was used to analyse the differential expression of lncRNA and mRNA.By calculating the Pearson correlation coefficient between differentially expressed lncRNA and mRNA,their co-expression was obtained.The positive co-expression of lncRNA-mRNA was screened by using a correlation coefficient.Go function and KEGG pathway enrichment of mRNA co-expressed with lncRNA were analysed to indirectly predict the function of lncRNA.Results A total of 202 differentially expressed exosome lncRNAs and 1014 exosome mRNA were screened from 32 healthy volunteers and 12 colorectal cancer patients.Ten pairs of lncRNA-mRNA co-expression were verified.Functional enrichment of mRNA co-expressed by lncRNA showed that differentially expressed exosome lncRNA may be closely related to B cell receptor signal pathway,NF-KB signal pathway,JAK-STAT signal pathway,antigen processing and presentation signal pathway and NOD-like receptor signal pathway activation.Conclusion In-depth exploration of differential expression,functional enrichment and pathway activation of exosome lncRNA in colorectal cancer is important to provide new ideas for the diagnosis and treatment of colorectal cancer.