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大豆异黄酮对去卵巢大鼠骨组织骨保护素及其配体mRNA表达的影响
引用本文:王强,王坤正,汪光晔.大豆异黄酮对去卵巢大鼠骨组织骨保护素及其配体mRNA表达的影响[J].中西医结合学报,2006,4(3):303-306.
作者姓名:王强  王坤正  汪光晔
作者单位:1. 西安交通大学第二医院骨科,陕西,西安,710004
2. 第二军医大学长海医院骨科,上海,200433
摘    要:目的:观察大豆异黄酮(soybean isoflavones,SI)对去卵巢大鼠骨组织中骨保护素(osteoprote-gerin,OPG)和骨保护素配体(osteoprotegerin ligand,OPGL)mRNA表达的影响,探讨SI防治绝经后骨质疏松(postmenopausal osteoporosis,PMO)的作用机制。方法:健康雌性SD大鼠30只,随机分为假手术组、模型组和SI治疗组。假手术组仅牵动卵巢,其余两组均行双侧卵巢切除术。SI治疗组造模后予以SI50 mg.kg-1.d-1灌胃,假手术组和模型组给予等量生理盐水灌胃,1次/d,每周用药6 d,共12周。12周后取大鼠L3~L6脊椎行骨密度检测。取大鼠左侧股骨提取总RNA,采用实时定量聚合酶链反应(real-timequantitative polymerase chain reaction,RTQ-PCR)技术检测OPG和OPGL mRNA的表达水平。结果:SI可以增加去卵巢大鼠的腰椎骨密度,上调去卵巢大鼠骨组织中OPG mRNA的表达水平,使OPGL mRNA/OPG mRNA比值下降,但对OPGL mRNA的表达则无明显影响。结论:SI防治PMO的效应可能与其调控OPG mRNA的表达及OPGL mRNA/OPG mRNA比值相关。

关 键 词:大豆异黄酮类  骨质疏松  绝经后  细胞因子  骨密度
文章编号:1672-1977(2006)03-0303-04
收稿时间:2005-06-01
修稿时间:2005年6月1日

Effects of soybean isoflavones on expression levels of osteoprotegerin and osteoprotegerin ligand mRNAs in bone tissues of ovariectomized rats
Qiang WANG,Kun-Zheng WANG,Guang-Ye WANG.Effects of soybean isoflavones on expression levels of osteoprotegerin and osteoprotegerin ligand mRNAs in bone tissues of ovariectomized rats[J].Journal of Chinese Integrative Medicine,2006,4(3):303-306.
Authors:Qiang WANG  Kun-Zheng WANG  Guang-Ye WANG
Institution:Department of Orthopaedics, Second Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710004, China. dadaol@126.com
Abstract:Objective: To investigate the effects of soybean isoflavones(SI) on expression levels of osteoprotegerin(OPG) and osteoprotegerin ligand(OPGL) mRNAs in bone tissues of ovariectomized rats,and to discuss the mechanism of soybean isoflavaones in preventing and treating postmenopausal osteoporosis(PMO).Methods: Thirty adult SD rats were randomly divided into 3 groups: sham-operation group,untreated group and SI-treated group.The rats in the last two groups were bilaterally ovariectomized.The bone density of L_(3) to L_(6) vertebrae was detected after 12-week intervention.Total RNA was extracted from femur head and the expression levels of OPG and OPGL mRNAs were examined by real-time quantitative polymerase chain reaction.Results: SI could increase the bone density of lumbar vertebrae in ovariectomized rats,up-regulate the expression level of OPG mRNA and down-regulate the ratio of OPGL mRNA/OPG mRNA,but exert no significant effect on the expression of OPGL mRNA.Conclusion: The therapeutic effects of SI on PMO may be related to regulating the expression levels of OPG and OPGL mRNAs,and the ratio of OPGL mRNA/OPG mRNA.
Keywords:soybean isoflavones  osteoporosis  postmenopausal  cytokines  bone density
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