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健脾活血方对酒精复合内毒素脂多糖诱导的肝损伤大鼠库普弗细胞活化信号通路的干预
引用本文:彭景华,方志红,崔剑巍,冯琴,许丽莉,顾宏图,胡义扬.健脾活血方对酒精复合内毒素脂多糖诱导的肝损伤大鼠库普弗细胞活化信号通路的干预[J].中西医结合学报,2007,5(3):302-306.
作者姓名:彭景华  方志红  崔剑巍  冯琴  许丽莉  顾宏图  胡义扬
作者单位:上海中医药大学曙光医院,上海中医药大学肝病研究所,上海,201203
基金项目:国家自然科学基金;上海市重点学科建设项目
摘    要:目的:观察健脾活血方对酒精复合内毒素脂多糖(lipopolysaccharide,LPS)诱导的肝损伤大鼠库普弗细胞活化信号通路的影响。方法:采用Lieber-Decarli酒精饮料饲养6周诱导的酒精性肝损伤模型,分设正常组,无酒精饮料组,酒精饮料组和酒精饮料加健脾活血方组,造模第3周起灌胃给药或蒸馏水至第6周末,各组再以LPS 10 mg/kg一次性灌胃,3.5 h后取材。检测血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)活性和肝组织甘油三酯(triglyceride,TG)含量;HE染色观察大鼠肝组织病理改变;CD68免疫组化观察库普弗细胞活化状态;ELISA法检测门脉血浆肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)含量;Western-blotting方法检测肝组织TNF-α、磷酸化的IκB(phosphorylation-IκB,P-IκB)、Toll样受体4(Toll-like receptor 4,TLR4)、CD68蛋白表达。结果:经健脾活血方干预后,大鼠肝脏组织病理损伤减轻,肝组织TG含量以及血清ALT活性和门脉血浆TNF-α含量下降;同时,大鼠肝脏TNF-α、P-IκB、TLR4和CD68蛋白表达明显减轻。结论:健脾活血方对酒精复合LPS诱导的肝损伤大鼠CD68、TLR4、P-IκB和TNF-α具明显抑制作用。

关 键 词:肝疾病  酒精性  内毒素  脂多糖  肿瘤坏死因子-α
文章编号:1672-1977(2007)03-0302-05

Effects of Jianpi Huoxue Decoction on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide
Jing-hua PENG,Zhi-hong FANG,Jian-wei CUI,Qin FENG,Li-li XU,Hong-tu GU,Yi-yang HU.Effects of Jianpi Huoxue Decoction on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide[J].Journal of Chinese Integrative Medicine,2007,5(3):302-306.
Authors:Jing-hua PENG  Zhi-hong FANG  Jian-wei CUI  Qin FENG  Li-li XU  Hong-tu GU  Yi-yang HU
Institution:Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Abstract:OBJECTIVE: To observe the effects of Jianpi Huoxue Decoction, a compound Chinese herbal medicine, on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide (LPS). METHODS: SD rats were divided into normal, control liquid diet, ethanol liquid diet and ethanol liquid diet plus Jianpi Huoxue Decoction group. Rats were administrated with Jianpi Huoxue Decoction or distilled water via gastrogavage for 4 weeks after administration with ethanol or control liquid diet for 2 weeks respectively. After that, rats in each group were stimulated with LPS via gastrogavage for 3.5 h and harvested. Alanine aminotransferase (ALT) in serum and triglyceride (TG) in liver were analyzed. Pathological changes in liver tissues were observed in HE staining section. Tumor necrosis factor-alpha(TNF-alpha) in portal vein plasma was assayed by ELISA. The protein expressions of CD68, Toll-like receptor 4 (TLR4), phosphorylation-I kappa B (P-I kappa B) and TNF-alpha in liver were evaluated with Western-blotting. RESULTS: After the treatment with Jianpi Huoxue Decoction, the pathologic changes in liver tissue were lightened, levels of ALT in serum, TG in liver and TNF-alpha in portal vein plasma were decreased, and the protein expressions of CD68, TLR4, P-IkappaB and TNF-alpha in liver were reduced. CONCLUSION: Jianpi Huoxue Decoction can inhibit Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and LPS.
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