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人子宫内膜异位症在位和异位内膜细胞原代培养及形态学观察
引用本文:俞超芹,石书芳,刘玉环,王瑞霞,宋艳华,俞瑾.人子宫内膜异位症在位和异位内膜细胞原代培养及形态学观察[J].中西医结合学报,2006,4(2):189-193.
作者姓名:俞超芹  石书芳  刘玉环  王瑞霞  宋艳华  俞瑾
作者单位:1. 第二军医大学长海医院中医科,上海,200433
2. 第二军医大学长海医院妇产科,上海,200433
3. 复旦大学妇产科医院,上海,200011
基金项目:国家自然科学基金资助项目(No.30371838)
摘    要:目的:探讨子宫内膜异位症(endometriosis,EM)异位子宫内膜细胞原代培养方法,并比较EM在位及异位子宫内膜细胞与正常对照在位子宫内膜细胞形态的差异。方法:采用改良EM细胞原代培养方法,免疫细胞化学法鉴定细胞类型,在光学显微镜及电子显微镜下观察细胞形态差异。结果:正常对照子宫内膜细胞及EM在位、异位子宫内膜细胞分离培养成功率分别为91.67%、93.75%和75.00%。EM异位、在位子宫内膜腺上皮细胞与正常在位子宫内膜腺上皮细胞大小相似,但EM异位子宫内膜腺上皮细胞染色质增多,核增大。EM异位子宫内膜间质细胞较EM在位及正常在位子宫内膜间质细胞小,且细胞膜表面有较多的微绒毛和胞浆突起。结论:注意取材方式,采用改良原代培养方法,可以提高EM异位子宫内膜细胞培养成功率。EM异位子宫内膜腺上皮细胞和间质细胞超微结构与正常妇女及EM在位子宫内膜细胞有显著不同。

关 键 词:子宫内膜异位症  原代培养  腺上皮细胞  间质细胞
文章编号:1672-1977(2006)02-0189-05
收稿时间:2005-07-01
修稿时间:2005年7月1日

Primary culture and morphologic observation of eutopic and ectopic endometrial cells from patients with endomehiosis
Chao-Qin YU,Shu-Fang SHI,Yu-Huan LIU,Rui-Xia WANG,Yan-Hua SONG,Jin YU.Primary culture and morphologic observation of eutopic and ectopic endometrial cells from patients with endomehiosis[J].Journal of Chinese Integrative Medicine,2006,4(2):189-193.
Authors:Chao-Qin YU  Shu-Fang SHI  Yu-Huan LIU  Rui-Xia WANG  Yan-Hua SONG  Jin YU
Institution:1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of Gynecology and Obstetrics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 3. Gynecology and Obstetrics Hospital, Fudan University, Shanghai 200011, China
Abstract:Objective: To explore the method of primary culture for endometriotic cells and to find out the differences in morphological manifestations among endometriotic cells and eutopic endometrial cells sampled from patients with endometriosis and endometriosis-free women. Methods: Endometriotic and eutopic endometrial cells were cultured by modified method of primary culture. The endometriotic cell types were observed and differentiated under optical and electron microscopes. Results: The success rates for culture of eutopic endometrial cells from endometriosis-free women and patients with endometriosis were 91.67% and 93.75% respectively. The success rate for culture of endometriotic cells was 75.00%. The size of endometriotic glandular cells was similar to those of eutopic endometrial glandular cells from endometriosis-free women and patients with endometriosis. The chromatin was manifold and the nucleus was augmented in the endometriotic glandular cells. The endometriotic stromal cells were smaller than the eutopic endometrial stromal cells from endometriosis-free women and patients with endometriosis. Many tiny villi and protuberances on plasma membrane could be seen in the endometriotic stromal cells. Conclusion: The success rate for culture of endometriotic cells can be elevated through improving the method of primary culture. The ultrastructures of endometriotic glandular and stromal cells are obviously different from those of eutopic endometrial glandular and stromal cells from endometriosis-free women and patients with endometriosis.
Keywords:endometriosis  primary culture  glandular cells  stromal cells
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