益气养阴法防治癌转移的实验研究

目的:研究益气养阴中药的抗肿瘤转移作用,探讨中药防治癌转移的作用机制.方法:50只小鼠分为益气组、养阴组、益气养阴组、化疗组和空白对照组,每组10只.以小鼠Lewis肺癌模型及B16黑色素瘤模型为实验观察对象.观察瘤重抑制率、肺部转移灶、瘤组织组织型纤溶酶原激活物和血栓烷B2的含量以及白细胞介素-2和肿瘤坏死因子-α的水平.结果:益气养阴中药对Lewis肺癌和B16黑色素瘤的瘤重抑制率均为42.9%.益气养阴中药能明显降低Lewis肺癌、B16黑色素瘤荷瘤小鼠瘤组织血栓烷B2和6-酮-前列腺素F1α的含量,提高组织型纤溶酶原激活物、白细胞介素-2及肿瘤坏死因子-α的水平.结论:益气养阴中药在抗癌转移过程中能最大限度地调动机体的抗癌能力,从而发挥抑制癌细胞转移的作用.

Experimental study on Qi-replenishing and Yin-nourishing in inhibiting tumor metastasis

OBJECTIVE: To study the effect of Qi-replenishing and Yin-nourishing in inhibiting tumor metastasis. METHODS: Fifty mice were randomly divided into replenishing Qi group, replenishing Yin group, replenishing Qi and Yin group, chemotherapy group and control group. Models of Lewis lung cancer and B(16) melanoma were observed in vivo. The tumor weight inhibiting rate, metastatic lung tumor, concentrations of t-PA and TXB(2) in tumor tissue and IL-2 and TNF-alpha in spleen were observed. RESULTS: The tumor weight inhibiting rates of replenishing Qi and Yin group for Lewis lung cancer and B16 melanoma were both 42.9%. Replenishing Qi and Yin could decrease the contents of TXB(2) and 6-keto-PGF(1alpha), and increase the levels of IL-2 and TNF-alpha. CONCLUSION: Replenishing Qi and Yin can promote the healthy energy to inhibiting tumor metastasis.