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消痰散结方对人胃癌MKN-45裸鼠移植瘤生长及细胞增殖相关蛋白表达的影响
引用本文:郭晓冬,魏品康.消痰散结方对人胃癌MKN-45裸鼠移植瘤生长及细胞增殖相关蛋白表达的影响[J].中西医结合学报,2007,5(4):432-436.
作者姓名:郭晓冬  魏品康
作者单位:1. 上海中医药大学岳阳中西医结合医院肿瘤科,上海,200437
2. 第二军医大学长征医院中医科,上海,200003
摘    要:目的:观察消痰散结方对裸鼠胃腺癌体内生长的抑制作用及其对胃癌组织中增殖细胞核抗原(proliferatingcell nuclear antigen,PCNA)和表皮生长因子受体(epidermal growth factor receptor,EGFR)表达的影响,探讨消痰散结方抑制胃癌细胞增殖的作用机制。方法:建立人胃腺癌MKN-45裸鼠原位种植模型,将45只模型鼠随机分为荷瘤对照组、中药组及化疗组,接种后次日分别给予生理盐水、中药灌胃及5-氟尿嘧啶(fluorouracil,5-FU)腹腔注射,6周实验结束后,观察移植瘤生长情况,免疫组化抗生物素蛋白链菌素-过氧化物酶(streptavidin peroxidase,SP)连接法检测胃癌组织PCNA及EGFR的阳性表达率,同时观察各组瘤组织的重量及病理学变化。结果:与对照组相比,消痰散结方可明显抑制裸鼠MKN-45胃腺癌移植瘤生长,下调裸鼠胃癌组织中PCNA和EGFR表达(P<0.05)。结论:消痰散结方可通过抑制胃癌细胞核中PCNA表达,干扰DNA合成并影响细胞表面生长因子受体表达,降低细胞增殖活性,抑制移植瘤生长。

关 键 词:胃肿瘤  中药  增殖细胞核抗原  表皮生长因子受体  裸小鼠
文章编号:1672-1977(2007)04-0432-05

Effect of Xiaotan Sanjie Recipe on growth of transplanted tumor and expressions of proliferating cell nuclear antigen and epidermal growth factor receptor in tissue of gastric carcinoma of nude mice
Xiao-dong GUO,Pin-kang WEI.Effect of Xiaotan Sanjie Recipe on growth of transplanted tumor and expressions of proliferating cell nuclear antigen and epidermal growth factor receptor in tissue of gastric carcinoma of nude mice[J].Journal of Chinese Integrative Medicine,2007,5(4):432-436.
Authors:Xiao-dong GUO  Pin-kang WEI
Institution:1. Department of Oncology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;2. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
Abstract:Objective: To observe the influence of Xiaotan Sanjie Recipe on growth of the transplanted tumor and expressions of proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGFR) in tissue of the gastric carcinoma of nude mice, and to explore the mechanism of Xiaotan Sanjie Recipe in restraining the multiplication of the stomach cancer.Methods: MKN-45 gastric carcinoma model in nude mice was established. Forty-five nude mice were randomly divided into control group, Xiaotan Sanjie group and 5-fluorouracil (5-FU) group. Drugs were given on the next day of innoculation. Mice in the control group were given normal saline, and mice in the Xiaotan Sanjie group were given Xiaotan Sanjie Recipe. Intraperitoneal injection of 5-FU was administered in the 5-FU group. The expressions of PCNA and EGFR were examined by using streptavidin peroxidase (SP) conjugate technique, and the tumor weight and pathological characteristics were also observed.Results: Compared with the control group, Xiaotan Sanjie Recipe significantly restrained the growth of the transplanted tumor, and reduced the expressions of PCNA and EGFR in tissue of the gastric carcinoma of nude mice (P<0.05).Conclusion: Xiaotan Sanjie Recipe can disturb the synthesis of DNA and reduce the proliferous activity of cancer cells by decreasing the expressions of PCNA and EGFR.
Keywords:stomach neoplasms  Chinese herbal drugs  proliferating cell nuclear antigen  epidermal growth factor receptor  nude mice
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