升清胶囊对豚鼠胆石病相关基因的影响

目的:探讨中药升清胶囊在分子层面防治胆石病的机制.方法:雌性豚鼠60只,随机分成正常组(喂正常饮食作为对照)、模型组(喂低蛋白饮食致石)和中药组(喂低蛋白饮食致石加中药升清胶囊治疗).6周后处死并取材,观察动物成石情况,检测肝组织胆红素尿苷二磷酸葡萄糖醛酸基转移酶(bilirubin UDP-glucuronosyltransferase, B-UGT)mRNA及胆固醇7α羟化酶(cholesterol 7α-hydroxylase, CYP7A1)mRNA表达.结果:各组豚鼠成石比例分别为正常组2/14、模型组为9/11、中药组为4/14,组间有显著差异(P＜0.05),正常组和中药组豚鼠的肝组织B-UGT mRNA及CYP7A1 mRNA表达明显高于模型组(P＜0.05).结论:中药升清胶囊可能是通过上调肝组织中B-UGT mRNA和CYP7A1 mRNA的表达,干预胆红素和胆固醇代谢,抑制致石性胆汁形成,起到预防胆结石的作用.

Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs

OBJECTIVE: To explore the molecular mechanisms of Shengqing Capsules in treating cholelithiasis. METHODS: Sixty female guinea pigs were randomized into 3 groups: group I (fed with normal diet), group II (fed with low-protein diet) and group III (fed with low-protein diet and Shengqing Capsules). After six-week feeding, the gallstone formation and the expressions of bilirubin UDP-glucuronosyltransferase (B-UGT) mRNA and cholesterol 7alpha-hydroxylase (CYP7A1) mRNA were observed. RESULTS: The proportions of stone-formed in groups I, II and III were 2/14, 9/12 and 4/14, respectively. There were significant differences among the three groups (P<0.05). The expressions of B-UGT and CYP7A1 mRNAs were higher in both group I and group III as compared with those in the group II (P<0.05). CONCLUSION: Shengqing Capsules can reduce the rate of stone-formation, which may be due to its interference of metabolism of bilirubin and cholesterol and up-regulation of the expressions of B-UGT and CYP7A1 mRNAs.