不同治法中药复方对H22肝癌小鼠瘤质量和体质量及免疫功能的影响

目的：比较不同治法的中药复方对H22肝癌小鼠瘤质量、体质量及免疫功能等的影响。方法：选择移植性肝癌H22小鼠为模型，观察不同治法的中药复方常规水煎剂的抑瘤作用，并通过检测各组小鼠T淋巴细胞增殖功能、自然杀伤细胞（naturalkiller，NK）杀伤功能、T淋巴细胞表型变化及7干扰素（interferon-7，IFN-7）和白细胞介素-4（interleukin-4，IL-4）含量来比较免疫功能状况。结果：以毒攻毒组小鼠瘤质量显著小于其他各组小鼠，抑瘤率达65．8%（P〈0．01）；其他4组抑瘤率在10.1％～17．1％之间。以毒攻毒组小鼠体质量明显下降，出现脱毛现象；扶正培本组小鼠毛发生长良好，活泼好动。以毒攻毒组小鼠T淋巴细胞转化刺激指数（stimulationindex，SI）增加显著（SI=4．34，P〈0.01），说明以毒攻毒组小鼠T细胞具有较强的增殖能力；其余各组小鼠sI均低于3，表明这些组的小鼠T细胞无明显增殖作用。清热解毒、活血化瘀和以毒攻毒组小鼠NK细胞百分数显著增高，分别为生理盐水组的5．05、4．07和5．17倍（P〈O．01）；扶正培本和化痰散结组小鼠NK细胞杀伤活性未见显著升高。IFN-7检测结果显示，以毒攻毒组小鼠T细胞分泌IFN-γ能力显著升高（P〈0．05）；IL-4检测结果显示，除扶正培本组外，清热解毒、活血化瘀、化痰散结和以毒攻毒组小鼠T细胞分泌IL-4的能力也均显著升高（P〈0．01）。结论：以毒攻毒法能有效抑制肿瘤细胞的生长，明显增强小鼠细胞和体液免疫功能；扶正培本法则有良好的强壮作用。

Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice

OBJECTIVE: To compare the influence of traditional Chinese compound recipes (TCCRs) with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. METHODS: H(22) cancer-bearing mice were chosen to observe the effects of TCCRs with different efficacy on tumor growth inhibition and detect the proliferation function of T lymphocytes, the activity of natural killer (NK) cells, the changes of T lymphocytes and the content of interferon-gamma (IFN-gamma)and interleukin-4 (IL-4). RESULTS: Tumor weight of H(22) cancer-bearing mice in Yidu Gongdu Recipe (YDGDR, a compound traditional Chinese herbal medicine using poison as an antidote for poison)-treated group was obviously lighter than that in the other TCCR-treated groups and the tumor inhibition rate in YDGDR-treated group was 65.76% (P<0.01). The tumor inhibition rates in other TCCR-treated groups were ranged from 10.1% to 17.1% . Body weight of mice in YDGDR-treated group was obviously decreased and depilation was observed at the same time. Pelage of mice in Fuzheng Peiben Recipe (FZPBR, a compound traditional Chinese herbal medicine for supporting the healthy energy)-treated group grew well, and behavior of the mice was active. Stimulation index (SI) of T lymphocyte transformation in YDGDR-treated group was obviously increased (SI=4.34, P<0.01), which showed the proliferation function of T lymphocyte was very strong. The SI of T lymphocyte transformation in the other groups was less than three, which showed the proliferation function of T lymphocytes was not significant. Compared with normal saline (NS)-treated group, percentages of NK cells in Qinre Jiedu Recipe (QRJDR, a compound traditional Chinese herbal medicine for clearing away heat and toxic substances)-treated, Huxue Huayu Recipe (HXHYR, a compound traditional Chinese herbal medicine for activating blood circulation to dissipate blood stasis)-treated and YDGDR-treated groups were obviously increased and 5.05, 4.07 and 5.17 times more than the NS-treated group, respectively (P<0.01). The activity of NK cells wasn't increased in the FZPBR-treated and HXHYR-treated groups. The production of IFN-gamma induced by T cells in YDGDR-treated group was obviously raised (P<0.05), and the production of IL-4 induced by T cells in QRJDR-treated, HXHYR-treated, Huatan Sanjie Recipe (a compound traditional Chinese herbal medicine for eliminating phlegm and resolving masses)-treated and YDGDR-treated groups was also raised obviously (P<0.01). CONCLUSION: YDGDR has a good effect of inhibiting tumor growth and can reinforce cellular and humoral immune function in tumor-bearing mice. FZPBR can strengthen the body.