四生汤对脾虚证小鼠免疫功能和抗应激能力的影响

目的：通过观察四生汤对生大黄致脾虚证小鼠一般生活状况、免疫功能、抗应激能力的影响，探讨其治疗脾虚证的作用机制。方法：将小鼠按照体质量随机分为正常组、脾虚模型组和低、中、高剂量四生汤组。采用造模与治疗同步的方式，即上午治疗组分别予高、中、低三个剂量的四生汤灌胃，脾虚模型组及正常组给予蒸馏水灌胃；下午除正常组予蒸馏水外，其余组给予生大黄粉灌胃，连续给药14d。观察小鼠的一般生活状况，并检测肛温、负重游泳时间、胸腺指数、脾脏指数和血清白细胞介素2（interleukin-2，IL-2）的含量。结果：灌服四生汤的小鼠较脾虚模型组精神好，稀便减少，食欲增强，体质量增长快；高、中剂量四生汤能明显提高脾虚证小鼠的肛温，改善畏冷；高、中、低剂量四生汤组的脾脏指数和胸腺指数与正常组比较差异无统计学意义（P〉0．05），而胸腺指数较脾虚模型组升高（P〈0．05）。高、中剂量的四生汤能明显提高脾虚证小鼠血清IL-2的含量（P〈0．05），明显延长脾虚证小鼠的负重游泳时间（P〈0．05）。结论：高、中剂量的四生汤具有良好的健脾益气作用，提高机体的免疫功能和抗应激能力是其主要的作用机制。

Effects of Sisheng Decoction on the immunity and anti-stress function in mice with spleen deficiency syndrome

OBJECTIVE： To study the possible mechanism of Sisheng Decoction on spleen deficiency syndrome via the observation of general conditions, immunity and anti-stress function in Dahuang （Radix et Rhizoma Rhei Palmati）-induced mice model. METHODS： Mice were randomized and grouped based on the body weight. The establishment of model and the treatment were done simultaneously. Except the mice in normal group, the decoction and the Dahuang powder were separately given in the morning and the afternoon for 14 d. The general condition of the mice, the rectal temperature, the time of burden swimming, the indexes of thymus and spleen and the interleukin-2 （IL-2） concentration in the serum were observed. RESULTS： The group treated with Sisheng Decoction showed better performance than that of the model group, including less stool, strong appetite and fast growth; the medium- and high- dose Sisheng Decoction increased the rectal temperature of mice. There was no statistical difference in the thymus and spleen indexes between the groups treated by Sisheng Decoction and the normal group （P~0.05）. The thymus index in groups treated by sisheng Decoction was signifi- cantly elevated as compared with the model group （P〈0.05）. The medium- and high-dose Sisheng Decoction significantly improved the concentration of IL-2 and prolonged the time of burden swimming, as compared to the model group （P〈0.05）, CONCLUSION： The medium- and high-dose Sisheng Decoction is good at invigorating spleen and replenishing qi. Qne of the possible mechanisms may be related with the improvement of the immunity and anti-stress function of the body.