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黄芪总苷和三七总皂苷配伍对小鼠缺血再灌注脑组织氧化应激的影响
引用本文:谭华,黄小平,邓常清.黄芪总苷和三七总皂苷配伍对小鼠缺血再灌注脑组织氧化应激的影响[J].中西医结合学报,2010,8(5):448-452.
作者姓名:谭华  黄小平  邓常清
作者单位:湖南中医药大学病理生理实验室,湖南长沙,401208
基金项目:湖南省教育厅资助项目 
摘    要:目的:研究黄芪总苷(astragalosides,AST)和三七总皂苷(Panax notoginseng saponins,PNS)配伍对C57BL/6小鼠脑缺血再灌注早期脑组织氧化应激的影响。方法:80只C57BL/6小鼠随机分为假手术组,模型对照组,高剂量AST和PNS配伍组(AST 220 mg/kg加PNS 230 mg/kg,每天1次,灌胃给药),中剂量AST和PNS配伍组(AST 110 mg/kg加PNS 115 mg/kg,每天1次,灌胃给药),低剂量AST和PNS配伍组(AST 55 mg/kg加PNS 57.5 mg/kg,每天1次,灌胃给药),AST组(110 mg/kg,每天1次,灌胃给药),PNS组(115 mg/kg,每天1次,灌胃给药)及依达拉奉组(4 mg/kg,每天2次,腹腔注射),连续治疗4 d。第4天给药后1 h,结扎双侧颈总动脉造成脑缺血20 min,然后再灌注60 min,取缺血脑组织制备组织匀浆,检测脑匀浆液丙二醛(malonaldehyde,MDA)、还原型谷胱甘肽(glutathione,GSH)、一氧化氮(nitric oxide,NO)含量和超氧化物歧化酶(superoxide dismutase,SOD)、一氧化氮合酶(nitric oxide synthase,NOS)活性。采用2×2析因设计方差分析方法分析110 mg/kg AST与115 mg/kg PNS配伍是否具有交互作用。结果:与假手术组比较,模型组脑组织MDA、NO含量和NOS活性显著升高(P〈0.01),SOD活性和GSH含量显著降低(P〈0.01)。与模型组比较,AST组MDA含量降低(P〈0.01),SOD活性升高(P〈0.05),GSH、NO含量和NOS活性无显著变化(P〉0.05);PNS(115 mg/kg)组MDA、NO含量显著降低(P〈0.01),而SOD、NOS活性和GSH含量无显著变化(P〉0.05);依达拉奉组脑组织MDA、NO含量和NOS活性降低(P〈0.01,P〈0.05),SOD活性升高(P〈0.05),GSH含量无显著变化(P〉0.05);高、中、低剂量AST和PNS配伍组MDA、NO含量和NOS活性降低(P〈0.01,P〈0.05),SOD活性和GSH含量升高(P〈0.01,P〈0.05)。析因设计方差分析表明,AST(110 mg/kg)和PNS(115 mg/kg)配伍对MDA、GSH、NO含量和SOD、NOS活性的影响有交互作用(P〈0.01)。结论:AST和PNS配伍对脑缺血再灌注早期的氧化应激损伤具有抑制作用,AST(110 mg/kg)和PNS(115 mg/kg)配伍对脑缺血再灌注后的氧化应激损伤具有协同的作用。

关 键 词:黄芪总苷  三七总皂苷  脑缺血  再灌注损伤  氧化应激  C57BL/6小鼠

Effects of astragalosides and Panax notoginseng saponins combination on oxidative stress of cerebral ischemic reperfusion injury in mice
Hua TAN,Xiao-ping HUANG,Chang-qing DENG.Effects of astragalosides and Panax notoginseng saponins combination on oxidative stress of cerebral ischemic reperfusion injury in mice[J].Journal of Chinese Integrative Medicine,2010,8(5):448-452.
Authors:Hua TAN  Xiao-ping HUANG  Chang-qing DENG
Institution:(Laboratory of Pathophysiology,Hunan University of Traditional Chinese Medicine,Changsha 410208,Hunan Province,China )
Abstract:Objective:To investigate the influence of astragalosides(AST) and Panax notoginseng saponins(PNS) combination on oxidative stress of brain tissues in C57BL/6 mice with cerebral ischemia-reperfusion injury. Methods:Eighty C57BL/6 mice were randomly divided into sham-operated group,untreated group,high-dose combination group(AST at a dose of 220 mg/kg plus PNS at a dose of 230 mg/kg),medium-dose combination group(AST at a dose of 110 mg/kg plus PNS at a dose of 115 mg/kg),low-dose combination group (AST at a dose of 55 mg/kg plus PNS at a dose of 57.5 mg/kg),AST(110 mg/kg) group,PNS(115 mg/kg) group and edaravone(4 mg/kg) group.AST and PNS were administered by gavage once daily for 4 days and edaravone was administered by intraperitoneal injection twice daily for 4 days.On the fourth day,bilateral common carotid arteries were ligated for 20 minutes to induce cerebral ischemia,followed by 60 minutes of reperfusion.Ischemic brain tissue was used to prepare tissue homogenate,then contents of malonaldehyde (MDA),glutathione(GSH) and nitric oxide(NO),and activities of superoxide dismutase(SOD) and nitric oxide synthase(NOS) in the homogenate were detected.Two×two analysis of variance of factorial design was used to analyze whether there was an interaction between AST at 110 mg/kg and PNS at 115 mg/kg. Results:Compared with sham-operated group,contents of MDA and NO,and activity of NOS in the untreated group were remarkably increased(P〈0.01).activity of SOD and content of GSH were decreased(P〈0.01).Compared with the untreated group,content of MDA in the AST group was decreased(P〈0.01) and activity of SOD was increased(P〈0.01).however,contents of GSH and NO and activity of NOS had no obvious changes(P〈0.05).Contents of MDA and NO in the PNS group was decreased as compared with the untreated group(P〈0.01),but activities of SOD and NOS and content of GSH had no changes(P〉0.05).Contents of MDA and NO and activity of NOS in brain tissues in the edaravone group were decreased (P〈0.01,P〈0.05).and activity of SOD was increased(P〈0.05),while content of GSH had no changes (P〉0.05).Contents of MDA and NO and activity of NOS in brain tissue in the AST and PNS combination groups were decreased(P〈0.01,P〈0.05),the activity of SOD increased(P〈0.01,P〈0.05),the content of GSH increased(P〈0.01,P〈0.05),and activity of SOD and content of GSH were increased (P〈0.01,P〈0.05).The results of analysis of variance of factorial design showed that there were interactions between AST(110 mg/kg) and PNS(115 mg/kg)(P〈0.01). Conclusion:Combination of AST(110 mg/kg) and PNS(115 mg/kg) has a restraint effect on the early oxidative stress injury in the brain after ischemia-reperfusion,and the combination has a synergistic or additive effect.
Keywords:astragalosides  Panax Notoginseng saponins  cerebral ischemia  reperfusion injury  oxidative stress  C57BL/6 mice
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