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补阳还五汤及其有效组分和三七总皂苷含药血浆抗血小板衍生生长因子诱导的血管平滑肌细胞增殖的作用
引用本文:李花,陈刚,邓常清.补阳还五汤及其有效组分和三七总皂苷含药血浆抗血小板衍生生长因子诱导的血管平滑肌细胞增殖的作用[J].中西医结合学报,2009,7(11):1078-1085.
作者姓名:李花  陈刚  邓常清
作者单位:1. 湖南中医药大学组织胚胎学教研室,湖南,长沙,410208
2. 湖南中医药大学病理生理学实验室,湖南,长沙,410208
基金项目:国家自然科学基金资助项目 
摘    要:目的:研究补阳还五汤及其有效组分和三七总皂苷(total Panax notoginseng saponins,TSPN)含药血浆对血小板衍生生长因子(platelet-derived growth factor,PDGF)诱导的血管平滑肌细胞(vascular smooth muscle cell,VSMC)增殖的影响。 方法:采用血浆药理学方法及PDGF-BB诱导VSMC增殖模型。以甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)法检测空白血浆对VSMC生长的影响和含药血浆对PDGF-BB诱导的VSMC增殖的半数抑制浓度,确定补阳还五汤及其有效组分和三七总皂苷用药浓度。MTT法和流式细胞术检测补阳还五汤及有效组分和TSPN、阿托伐他汀对VSMC增殖活性及细胞周期的影响。 结果:PDGF-BB剌激后,VSMC增殖活性显著增强,G0/G1期细胞数减少,G2/M期和S期细胞数增多。与PDGF-BB剌激组比较,补阳还五汤、有效组分生物碱和苷、TSPN、阿托伐他汀等含药血浆均可抑制PDGF-BB诱导的VSMC增殖,增加G0/G1期细胞数,减少G2/M期和S期细胞数。 结论:补阳还五汤及其有效组分和TSPN均可抑制PDGF-BB诱导的VSMC增殖。生物碱和苷可能为补阳还五汤中抗VSMC增殖的主要药效物质,补阳还五汤及其有效组分生物碱和苷具有与阿托伐他汀、TSPN相似的抗VSMC增殖的作用。

关 键 词:补阳还五汤  生物碱    三七总皂苷  血浆药理学  血管平滑肌细胞  细胞增殖

Effects of plasma containing Buyang Huanwu Decoction and its effective components and total Panax notoginseng saponins on vascular smooth muscle cell proliferation induced by platelet-derived growth factor
Hua LI,Gang CHEN,Chang-qing DENG.Effects of plasma containing Buyang Huanwu Decoction and its effective components and total Panax notoginseng saponins on vascular smooth muscle cell proliferation induced by platelet-derived growth factor[J].Journal of Chinese Integrative Medicine,2009,7(11):1078-1085.
Authors:Hua LI  Gang CHEN  Chang-qing DENG
Institution:1.Department of Histology and Embryology, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China;2.Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China)
Abstract:Objective: To explore the effects of plasma containing Buyang Huanwu Decoction (BYHWD), a compound of traditional Chinese medicine, and its effective components (alkaloids and glycosides) and total Panax notoginseng saponins (TSPN) on vascular smooth muscle cell (VSMC) proliferation induced by platelet-derived growth factor (PDGF) in vitro.
Methods: VSMC proliferation was induced by incubating VSMCs with PDGF-BB. Effects of different concentrations of blank plasma on the VSMC proliferation and 50% inhibitory concentrations of plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin were detected by using methyl thiazolyl tetrazolium (MTT) method to determine the concentrations of plasma containing drugs for the following experiments. Then, MTT and flow cytometry methods were used to detect the effects of plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin on VSMC proliferative activity and cell cycles.
Results: VSMC proliferative activity was enhanced obviously after PDGF-BB stimulation. The cell number in G0/G1 phase was decreased and the cell quantity in G2/M and S phases was increased following PDGF-BB stimulation. Compared with PDGF-BB stimulation group, VSMC proliferation induced by PDGF-BB in plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin groups was inhibited. In all plasma containing drug components groups, the cell number of G0/G1 phase was higher and the cell quantity of G2/M and S phases was lower than those in the PDGF-BB stimulation group.
Conclusion: BYHWD and its alkaloids and glycosides have similar roles of resisting VSMC proliferation to TSPN and atorvastatin. Alkaloids and glycosides may be the major effective substances of anti-VSMC proliferation in BYHWD.
Keywords:Buyang Huanwu Decoction  alkaloids  glycoside  total Panax notoginseng saponins  plasmapharmacology  vascular smooth muscle cell  cell proliferation
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