复方银思维对散发性老年痴呆模型大鼠学习记忆能力的影响及其海马神经元超微形态学基础（英文）

目的：探讨中药复方银思维对散发性老年痴呆（sporadic Alzhei mer disease,SAD）模型大鼠空间学习记忆功能障碍的作用及其海马神经元超微形态学基础。方法：采用侧脑室注射链脲佐菌素方法制备SAD模型。实验大鼠分为假手术组、模型组、多奈哌齐组和银思维低、中、高剂量组共6组。于造模后第21天开始治疗,各治疗组给予相应的药物灌胃,模型组及假手术组给予等体积的双蒸水灌胃,各组均每日给药1次,共2个月。治疗结束后,以Morris水迷宫定位航行实验及空间探索实验进行大鼠空间学习记忆能力测试,测试完毕处死大鼠并取出海马组织,电子显微镜观察海马神经元超微结构。结果：在定位航行实验中,多奈哌齐组以及银思维低、中、高剂量组与模型组相比,平均逃避潜伏期及总游泳距离均显著缩短（P〈0.05或P〈0.01）。在空间探索实验中,与模型组相比,各治疗组在目标象限活动时间明显延长（P〈0.05或P〈0.01）。在多数测试时间段中,多奈哌齐组与银思维低、中、高剂量组组间比较差异均无统计学意义。脑组织海马神经元超微结构观察也证实了药物干预的作用。结论：中药复方银思维能明显改善SAD模型大鼠的空间学习记忆功能障碍。其作用的超微形态学基础可能与保护海马神经元微管结构,防止轴突损伤有关。

Effects of Chinese herbal medicine Yinsiwei compound on spatial learning and memory ability and the ultrastructure of hippocampal neurons in a rat model of sporadic Alzheimer disease

Objective: To study the effects of Chinese herbal medicine Yinsiwei compound(YSW)on spatial learning and memory ability in rats with sporadic Alzheimer disease(SAD)and the ultrastructural basis of the hippocampal neurons.Methods: A rat model of SAD was established by intracerebroventricular injection of streptozotocin.The rats were divided into six groups,sham-operation group,model group,donepezil control group,and YSW low,medium and high dose groups.Drug interventions were started on the2]st day after modeling and each treatment group was given the corresponding drugs by gavage for two months.Meanwhile,the model group and the sham-operation group were given the same volume of distilled water by gavage once a day for two months.The Morris water maze was adopted to test spatial learning and memory ability of the rats.The place navigation test and the spatial probe test were conducted.The escape latency,total swimming distance and swimming time in the target quadrant of the rats were recorded.Also,the hippocampus tissues of rats were taken out and the ultrastructure of hippocampus neurons were observed by an electron microscope.Results: In the place navigation test,compared with the model group,the mean escape latency and the total swimming distance of the donepezil group and the YSW low,medium and high dose groups were significantly shortened(P<0.05 or P<0.01).In the space probe test,the swimming time of each treatment group in the target quadrant was significantly longer than that of the model group(P<0.05 or P<0.01).For most of the test period,the donepezil group had no significant change compared with the YSW low,medium and high dose groups,respectively.The ultrastructure of the hippocampus neurons under the electron microscope also confirmed the efficacy of the drug treatment.Coaclusion.Chinese herbal medicine YSW compound can improve spatial learning and memory impairment of rats with SAD.The ultrastructural basis may be that it can protect the microtubule structures of hippocampal neurons and prevent nerve axons from being damaged.Keywerds.Alzheimer disease; drugs,Chinese herbal; maze learning; learning disorders;memory disorders; hippocampus; animal experimentation; rats