丹参多酚酸盐对人血管内皮细胞迁移的影响

目的 观察丹参多酚酸盐对单核细胞诱导的内皮细胞迁移的影响，探讨丹参多酚酸盐促血管生成的机制。方法 采用单核细胞-内皮细胞联合培养，观察丹参多酚酸盐对单核细胞诱导的内皮细胞迁移的影响。分别采用酶联免疫吸附测定法和逆转录聚合酶链反应法检测丹参多酚酸盐干预后单核细胞分泌血管内皮细胞生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)水平及其mRNA的变化。结果 迁移实验结果示经丹参多酚酸盐处理后内皮细胞的迁移数增加，与对照组比较，有显著性差异。经丹参多酚酸盐处理后，单核细胞VEGFmRNA和bFGF mRNA均有升高，VEGF和bFGF蛋白水平也有上升。结论 丹参多酚酸盐促进单核细胞诱导的内皮细胞迁移；其促进单核细胞合成和分泌VEGF及bFGF，可能是该药物促内皮细胞迁移的作用机制。

Effect of salvianolate on migration of human vascular endothelial cells

ObjectiveTo study the effect of salvianolate on th e migration of endothelial cells induced by monocytes. MethodsTrans well-boydom system was used to test the migration of endothelial cells induced by monocytes. Enzyme-linked immunosorbent assay (ELISA) and RT-polymerase chai n reaction (RT-PCR) method were used to determine the effect of salvianolate on the expression of vascular endothelial growth factor (VEGF) and basic fibroblas t growth factor (bFGF)and their mRNA of monocytes. ResultsThe mig ration of endothelial cells induced by monocytes was facilitated by salvianolate , compared with the control. After the monocytes were treated by salvianolate fo r 24 hours, the expression of mRNA of VEGF and bFGF was significantly up-regula ted, and the expression of VEGF and bFGF was increased. Conclusion The facilitation of salvianolate on the migration of endothelial cells induced by monocytes was observed. Salvianolate stimulates the expression of VEGF and b FGF and their mRNA of monocytes, and salvianolate may have the role of inducing migration of endothelial cells by working on these two factors.