肺岩宁方对人脐静脉内皮细胞增殖、迁移及管腔形成的影响

目的：观察中药肺岩宁方含药血清对人脐静脉内皮细胞（human umbilical vein endothelial cell，HUVEC）增殖、迁移及管腔形成的影响。方法：采用新鲜产后脐带，分离HUVEC。SD大鼠灌胃肺岩宁方制备肺岩宁方含药血清，采用人肺腺癌细胞A549制备条件培养液。用磺酰罗丹明B（sulforhodamineB，SRB）法观察肺岩宁方含药血清对HUVEC、人肺腺癌细胞A549和A549细胞条件培养液诱导HUVEC增殖的抑制作用；采用Boyden Chamber Transwell法检测其对HUVEC细胞迁移的影响；管腔形成实验评价其对HUVEC形成管腔能力的影响。结果：与同浓度对照血清相比，肺岩宁方含药血清能够抑制HUVEC及肺癌细胞条件培养液诱导的内皮细胞的增殖（P〈0．01，P〈0．05），高浓度肺岩宁方含药血清能够抑制肿瘤细胞A549的体外增殖，肺岩宁方含药血清（12．5％～37．5％）在体外能抑制20％胎牛血清诱导的内皮细胞的迁移（P〈0．05，P〈0．01）以及初级管腔的形成。结论：肺岩宁方具有明显的抑制新生血管生成的作用，这可能是肺岩宁方抗肺癌侵袭转移的机制之一。

Inhibitory effects of Feiyanning Decoction on proliferation,migration and tube formation of human umbilical vein endothelial cells in vitro

Objective： To observe the inhibitory effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine for replenishing qi, nourishing essence, and diminishing stagnation by detoxification, on proliferation, migration and tube formation of human umbilical vein endothelial cells （HUVECs）. Methods ： HUVECs were isolated from the new-born umbilical core of human. Serum containing Feiyanning was prepared in SD rats by oral gavage of the Feiyanning Decoction for three days. The inhibitory effects of different contents of serum containing Feiyanning on the proliferation of HUVECs, human lung adenocarcinoma cell A549, and HUVECs cultured with A549-conditioned media were observed by sulforhodamine B （SRB） method; the effects on migration of HUVECs were inspected by using Boyden Chamber Transwell method, and the effects on tube formation of HUVECs were evaluated by cavity forming experiment. Results： Serum containing Feiyanning at a concentration of 25%, 37.5% or 50% inhibited the proliferation of both the HUVECs and the HUVECs cultured with A549-conditioned media （P〈0.01, P〈0.05）. High concentration serum containing Feiyanning inhibited the proliferation of A549 cells. Sera containing Feiyanning at concentrations of 12.5% to 37.5% inhibited the migration of HUVECs induced by 20% fetal bovine serum （P〈0.05, P〈0.01） and the formation of the primary tube of HUVECs. Conclusion： Feiyanning Decoction can inhibit the angiogenesis of HUVECs, which may be one of its mechanisms in inhibiting the invasion metastases of lung cancer .