姜黄素抑制晶状体上皮细胞增殖的信号转导机制

目的:探讨姜黄素(curcumin,Cur)抑制重组人表皮生长因子(recom binanthuman epidermal growth factor,rhEGF)诱导的牛晶状体上皮细胞(lens epithelial cell,LEC)增殖的信号转导机制。方法:采用荧光分光光度法检测Cur作用后LEC内游离Ca2 浓度;应用放射免疫分析法检测Cur作用后LEC内cAMP和cGMP含量的变化。结果:经50μg/LrhEGF作用后,LEC内游离Ca2 浓度明显升高,Cur可使LEC内游离Ca2 浓度进一步升高。经50μg/LrhEGF作用后,LEC内cAMP浓度明显下降,cGMP浓度明显升高;而Cur则可使rhEGF作用后的LEC内cAMP浓度明显升高,cGMP浓度明显降低。结论:Cur可抑制rhEGF诱导的LEC增殖,其抑制细胞增殖的作用可能是通过多条信号转导途径来实现的。

Signal transduction mechanism of curcumin in inhibiting the proliferation of bovine lens epithelial cell induced by recombinant human epidermal growth factor

OBJECTIVE: To investigate the signal transduction mechanism of curcumin in inhibiting the proliferation of bovine lens epithelial cell (LEC) induced by recombinant human epidermal growth factor (rhEGF). METHODS: There were three groups in this experiment, which were normal control group, untreated group and curcumin-treated group. Proliferation of LEC was induced by rhEGF (50 microg/L). The concentration of intracellular Ca(2+) (Ca(2+)](i)) in LEC was measured with Fura-2/AM by spectrofluorimetry. The contents of intracellular cAMP and cGMP were assayed by radioimmunoassay. RESULTS: The Ca(2+)]i in LEC was obviously increased in the untrated group as compared with that in the normal control group (P<0.01), and the Ca(2+)](i) in LEC in the curcumin-treated group was highest among three groups (P<0.01). The content of intracellular cAMP in LEC was decreased while the content of intracellular cGMP was obviously increased in the untreated group as compared with those in the normal control group (P<0.01). The content of intracellular cAMP in LEC was higher in the curcumin-treated group than that in the untreated group, while the content of intracellular cGMP was lower than that in the untreated group (P<0.01). CONCLUSION: The antiproliferation effects of curcumin on LEC may relate to the regulations of multiple processes of signal transduction.