几种水稻田土壤微生物总DNA提取方法的比较

目的：使人们在提取土壤总DNA时，可以根据不同的要求选用不同的方法。方法：选用了5种现在较常用的土壤微生物总DNA提取方法从水稻田土壤中提取总DNA，并对5种方法提取的DNA的质量和数量进行比较评价。结果：5种方法都可以从土壤中提取到长度大于48kb的DNA片段，但不同方法提取的DNA的产量存在明显差异。土壤总DNA都不需要纯化就可以用于PCR扩增，使用细菌16SrDNA基因的引物可以扩增得到相应的片段。结论：Bourrain法和Martin-Laurent法可以较快地提取出DNA，Martin-Laurent法提取的DNA的量最多，Tiedje法和Janssen法提取的DNA片段最大，Janssen法提取的DNA纯度最高，Reddy法提取的DNA的完整性和PCR扩增效果最好。

Comparison of Methods of DNA Extraction from Paddy Soil

Objective: To make it possible that when DNA is extracted from soil,different methods could be chosen according to different demands. Methods: In this experiment,five different methods commonly used to extract DNA from soil were chosen to extract DNA from paddy soil.Then the quality and quantity of DNA were compared to estimate the five methods. Results: DNA extracted by five methods were all greater than 48kb in size,but the yields were different.Subsequently,they were directly used as templates for PCR amplification without purification,using primers of the bacterial 16S rDNA gene. Conclusion: Bourrain method and Martin-Laurent method can generate DNA quicker than other methods.Martin-Laurent method can generate the largest amount of DNA.Tiedje method and Janssen method can generate the largest DNA fragments in size.Janssen method can generate the purest DNA.Reddy method can generate DNA in highest integrity which was the most suitable for PCR amplification.