虾青素对小鼠急性乙醇肝损伤的保护作用

目的:研究虾青素对乙醇所致小鼠急性化学性肝损伤的保护作用。方法:雄性小鼠60只,随机分为正常对照组、急性乙醇肝损伤模型组、联苯双酯阳性对照组(15 mg/kg)以及虾青素低、中、高剂量组(10,15,20 mg/kg)共6组。测定并比较各组小鼠肝脏系数,血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-px)活性与丙二醛(MDA)含量;测定肝组织中SOD,GSH-px活性,MDA含量以及组织病理系数。结果:各剂量虾青素均能升高急性乙醇肝损伤小鼠血清与肝组织中SOD,GSH-px活性(P<0.01),降低血清ALT,AST活性(P<0.01),降低血清与肝组织MDA含量(P<0.01),并能不同程度地改善肝脏病理组织损伤。结论:虾青素对乙醇所致急性肝损伤具有预防性保护作用。

Protective effects of astaxanthin on acute alcohol liver injury in mice

Objective: To study the protective effects of astaxanthin against the acute alcohol liver injury in mice and research its possible mechanism for offering scientific basis of its health function.Methods: Randomly to distribute 60 male mices to control group,acute alcohol liver injury model group,DDB group(15 mg/kg) and low,middle,high dose group s of astaxanthin(10,15,20 mg/kg),and to determine and compare liver index between groups,with serum alanine transaminase(ALT),aspartate transaminase(AST),superoxide dismutase(SOD),glutathione perioxidase(GSH-px) activities,malondialdehyde(MDA) content,liver SOD,GSH-px activities,MDA content and liver tissue pathology index to be assayed. Results: Treatment with different dose astaxanthin could significantly increase serum and liver tissue SOD,GSH-px activities(P<0.01),decrease serum ALT,AST levels(P<0.01) and MDA content in liver tissue(P<0.01).Pathology index showed that astaxanthin could ameliorate the liver injury in different degree. Conclusion: Astaxanthin showed protective effects on the acute alcohol liver injury induced by alcohol in mice.