| 人脐带间质干细胞的CM-Dil标记及其移植在急性肾损伤大鼠体内的示踪研究 |
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| 引用本文： | 徐会涛,周颖,王冰莹,毛飞,徐会娟,许文荣,朱伟,钱晖.人脐带间质干细胞的CM-Dil标记及其移植在急性肾损伤大鼠体内的示踪研究[J].江苏大学学报(医学版),2012,22(3):209-212. |
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| 作者姓名： | 徐会涛 周颖 王冰莹 毛飞 徐会娟 许文荣 朱伟 钱晖 |
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| 作者单位： | 江苏大学基础医学与医学技术学院,江苏镇江,212013 |
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| 基金项目： | 国家自然科学基金资助项目，江苏省博士研究生创新基金资助项目，江苏大学高级人才启动基金资助项目，江苏大学科技创新团队和拔尖人才项目 |
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| 摘 要： | 目的:探讨人脐带间质干细胞(human umbilical cord mesenchymal stem cells,hucMSCs)用CM-Dil染料标记之后生物学特性及体内定位示踪情况。方法:采用脐带组织块贴壁培养法分离人脐带间质干细胞,用CM-Dil染料标记第3代hucMSCs;采用细胞计数法分析CM-Dil标记后hucMSCs的生长变化情况;通过活体成像仪观察CM-Dil染料标记的hucMSCs移植于急性肾损伤大鼠后的体内定位。结果:CM-Dil染料标记hucMSCs的效率很高;标记后的hucMSCs生长增殖能力没有发生明显变化;体内示踪可以观察到标记的hucMSCs。结论:CM-Dil标记不影响hucM-SCs的生长增殖特征,并且能够在体内示踪,为后续研究提供了实验基础。
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| 关 键 词： | 人脐带间质干细胞 CM-Dil染料 体内示踪 急性肾损伤 |
Human umbilical cord mesenchymal stem cells labeled with CM-Dil and transplanted in acute kidney injury rats for tracer study |
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| XU Hui-tao
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ZHOU Ying
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WANG Bing-ying
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MAO Fei
,
XU Hui-juan
,
XU Wen-rong
,
ZHU Wei
,
QIAN Hui.Human umbilical cord mesenchymal stem cells labeled with CM-Dil and transplanted in acute kidney injury rats for tracer study[J].Journal of Jiangsu University Medicine Edition,2012,22(3):209-212. |
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| Authors: | XU Hui-tao ZHOU Ying WANG Bing-ying MAO Fei XU Hui-juan XU Wen-rong ZHU Wei QIAN Hui |
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| Institution: | (School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
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| Abstract: | Objective: To investigate whether the characteristics of human umbilical cord mesenchymal stem cells(hucMSCs) labeled with CM-Dil will be changed;and if CM-Dil labled hucMSCs can be traced in vivo.Methods: HucMSCs were isolated from human umbilical cord.The hucMSCs of passage 3 were selected for labeling in vitro.Using cell counting method to analyse growth curves of CM-Dil labeled hucMSCs;observed CM-Dil labeled hucMSCs in vivo with imaging instrument.Results: The efficiency of CM-Dil dye-labeled hucMSCs was high.The proliferation of labeled hucMSCs had no obvious change.CM-Dil dye-labeled hucMSCs can be observed in vivo.Conclusion: CM-Dil labeled hucMSCs can be traced in vivo. |
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| Keywords: | hucMSCs CM-Dil trace in vivo acute kidney injury |
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